Objective To study chemical constituents from fruits of Gymnocladus chinensis. Methods The compounds were isolated by various chromatographic techniques and their structures were elucidated on the basis of spectral analyses. Results Ten compounds were isolated from the EtOAc extract and were identified as 2-methyl pentyl (2E, 6S)-6-hydroxy-2, 6-dimethyl-2, 7-octadienoate (1), (2E, 6S)-6-?-L-arabino pyranosyloxy-2, 6-dimethyl-2, 7- octadienoic acid (2), ethyl (2E, 6S)-6-hydroxy-2, 6-dimethyl-2, 7-octadienoate (3), 5, 2?, 5?-trihydroxy-3, 7, 4?- trimethoxyflavone (4), (2R, 3R)-3, 3?, 5, 5?, 7-pentahydroxyflavanone (5), 2?, 4?-dihydroxy-4-methoxychalcone (6), kaempferol (7), apigenin (8), 4?, 7-dihydroxyflavone (9), and 5, 7, 4?-trihydroxy-3?-methoxyflavone (10). Conclusion Compound 1 is a new compound, compound 3 is firstly obtained from natural source, and compounds 4 – 10 are isolated from G. chinensis for the first time. Furthermore, the assignments of 13C-NMR data of compounds 3 and 4 are reported in this paper for the first time.

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