RP-HPLC Determination of 1,3-Dideoxygalactonojirimycin in Bombycis Faeces
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RP-HPLC Determination of 1,3-Dideoxygalactonojirimycin in Bombycis Faeces
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Abstract:
Objective To establish a simple and rapid method for the determination of 1,3-dideoxygalactonojirimycin in Bombycis Faeces, a potent glucosidase inihibitor, by HPLC. Methods A RP-HPLC method with fluorescence detection has been developed. Results The HPLC method developed in this research has a good reliability including accuracy and precision. The detection limit was less than 72 ng. Conclusion This method is sufficiently sensitive for determining 1,3-dideoxygalactonojirimycin in Bombycis Faeces and other related products.
QI Hui, ZHAO Hui, DING Zhen-ying, ZHU Yuan-yuan. RP-HPLC Determination of 1,3-Dideoxygalactonojirimycin in Bombycis Faeces[J]. Chinese Herbal Medicines (CHM),2011,3(2):156-158
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Manuscript received: September 14,2010
Manuscript revised: March 01,2011
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