醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

Archive > Volume 4 Issue 3 > 2012,4(3):230-236. DOI:10.3969/j.issn.1674-6384.2012.03.010 Prev Next

Original articles

Simultaneous Determination of Seven Alkaloids in Fufang Zhenzhu Tiaozhi Capsule by HPLC Coupled with DAD

  • Article
  • Figures
  • Metrics
  • Preview PDF
  • Reference
  • Related
  • Cited by
  • Materials
    摘要:


    Abstract:
    Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids (magnoflorine, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine) in Fufang Zhenzhu Tiaozhi Capsule. Methods Chromatography was performed on a Dionex Acclaim C18 column (250 mm × 4.6 mm, 5.0 μm) at 30 ℃. The mobile phase was composed of acetonitrile-potassium dihydrogen phosphate solution (0.015 mol/L, 40:60, including 1.7 g/L sodium dodecyl sulfate and phosphoric acid used to regulate pH value to 3.0), with a flow rate of 1.0 mL/min. The detection wavelength was 270 nm. Results The calibration curves of magnoflorine, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine were linear in the range of 1.07－10.65, 0.78－7.55, 0.75－7.50, 1.60－15.95, 2.69－26.85, 2.31－23.10, and 6.04－60.40 mg/mL. The average recoveries of magnoflorine, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine were 101.0%, 101.2%, 100.1%, 100.0%, 100.1%, 101.1%, and 99.7%, respectively. Conclusion The method could be used for the quantitative determination of the preparation.

    關(guān)鍵詞:


    Keywords:


    Project Supported:
    Cooperation Project in Industry, Education and Research of Guangdong Province and Ministry of Education of China (2009B090300349) and a Cooperation Project of National Science Foundation of Guangdong Province (10351022401000000)

    Clc Number:

WeChat

Mobile website