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Archive > Volume 10 Issue 4 > 2018,10(4):416-423. DOI:10.1016/j.chmed.2018.08.007 Prev Next

響應(yīng)面優(yōu)化酶法提取石榴皮多糖及其抗氧化活性研究

Original articles

Optimization of enzyme assisted extraction of polysaccharides from pomegranate peel by response surface methodology and their antioxidant potential

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    摘要:
    目的 采用響應(yīng)面法優(yōu)化石榴皮多糖的酶法提取工藝，并對(duì)石榴皮多糖體外抗氧化活性進(jìn)行研究，為藥物制劑和功能性食品尋找新的生物成分。方法 采用RSM Box-Behnken設(shè)計(jì)法，考察酶解時(shí)間、液料比、加酶量對(duì)石榴皮多糖提取率的影響。用酶標(biāo)儀測(cè)定DPPH自由基清除作用、羥自由基清除作用、超氧陰離子自由基清除活性和還原力測(cè)定。結(jié)果 最佳提取條件為：酶解溫度為55℃，pH為5，酶解時(shí)間為88 min，液料比為22:1mL/g，加酶量為0.93%。在最佳提取條件下，石榴皮多糖得率為（22.31±0.07）%，與Box-Behnken設(shè)計(jì)模型的預(yù)測(cè)值22.35%很好地吻合。石榴皮多糖對(duì)DPPH（1,1-二苯基-2-吡啶基肼）自由基清除、羥自由基清除、超氧陰離子自由基清除和還原能力有明顯的抗氧化作用。結(jié)論 建議采用優(yōu)化的酶解輔助方法提取石榴皮多糖，該法制得的石榴皮多糖可作為一種良好的抗氧化劑。

    Abstract:
    Objective To optimize the enzymatic extraction conditions of polysaccharides from pomegranate peel using response surface methodology (RSM), and invest the anti-oxidant activity of pomegranate peel polysaccharide in vitro for seeking novel biological components used as pharmaceutical products and functional foods. Methods Effects of enzymolysis time, ratio of water to raw material, and the dosage of cellulase on the extraction yield of pomegranate peel polysaccharide were fully examined by Box-Behnken design of RSM. DPPH radical scavenging activity, hydroxyl radicals scavenging activity, superoxide anion radical scavenging activity, and reducing power assay were measured by microplate reader. Results The optimal extraction conditions were as follows: enzymolysis temperature 55 ?C, pH 5.0, enzymolysis time 88 min, ratio of water to raw material 22:1 mL/g, and dosage of cellulose 0.93%. Under the optimal extraction conditions, the yield of pomegranate peel polysaccharide was (22.31 ± 0.07)%, which was well matched with the predicted value 22.35% of the Box-Behnken design model. Also, pomegranate peel polysaccharide demonstrated appreciable anti-oxidant potential on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging and reducing powerin vitro. Conclusions An optimized enzymolysis assisted method was proposed for the extraction of pomegranate peel polysaccharide, which can be used as a good anti-oxidant.

    關(guān)鍵詞:
    抗氧化;提取 ;多糖;石榴皮;響應(yīng)面

    Keywords:


    Project Supported:
    National Natural Science Foundation of China (No. 31301598), the Fundamental Research Fund for the Central Universities of China (No. GK201803086)

    Clc Number:

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