醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

Archive > Volume 11 Issue 1 > 2019,11(1):39-44. DOI:10.1016/j.chmed.2018.10.003 Prev Next

IMPX977對雷特綜合征相關(guān)的MeCP2靶基因的積極影響

Original articles

Positive effects of low dose IMPX977 on Rett syndrome related MeCP2 targeted-genes

  • Article
  • Figures
  • Metrics
  • Preview PDF
  • Reference
  • Related
  • Cited by
  • Materials
    摘要:
    為了探討IMPX977對雷特綜合征相關(guān)的MeCP2靶基因的影響以及能否作為治療雷特綜合征的候選藥物，我們結(jié)合轉(zhuǎn)錄組測序、qPCR等實(shí)驗(yàn)對該問題進(jìn)行了分析。我們將18只正常的SD大鼠分為正常組、低劑量組(10 mg/kg)和高劑量組(30 mg/kg)（n=6）。并從各組中分別提取高質(zhì)量的RNA用于轉(zhuǎn)錄組測序，對測序結(jié)果分析獲得的差異基因用GO和KEGG分析軟件分別對其富集分析。結(jié)果顯示較多的差異基因被富集到與神經(jīng)功能、鈣離子調(diào)控、G蛋白偶聯(lián)信號通路等相關(guān)的生物學(xué)過程。同時(shí)，在低劑量組(10 mg/kg)篩選的差異基因中鑒定了6個(gè)MeCP2的靶基因，在高劑量組(30 mg/kg)沒有發(fā)現(xiàn)相似的結(jié)果。同時(shí)，我們發(fā)現(xiàn)低劑量組的IMPX977(10 mg/kg)對雷特綜合征相關(guān)的MeCP2靶基因表現(xiàn)出積極的影響，這說明IMPX977有望成為治療雷特綜合征的候選藥物。

    Abstract:
    Objective To investigate the effect of IMPX977 on MeCP2 targeted-genes and the feasibility of IMPX977 acting as a therapeutic candidate drug for Rett syndrome by genomewide transcription profiling. Methods Rats’ cortex of control group, IMPX977-treated low-dose group (10 mg/kg), and IMPX977-treated high-dose group (30 mg/kg) were collected and RNA was extracted from the tissues. Then, RNA was subjected to RNA-sequencing. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) were used in functional enrichment analysis of differentially expressed genes. Results Six MeCP2 targeted-genes were identified in the low/control categories, but not in the high/control categories. Conclusion Low-dose treatment of IMPX977 (10 mg/kg) showed a positive effect on MeCP2 targeted-genes and it may serve as a drug candidate for Rett syndrome therapy with proper dosage.

    關(guān)鍵詞:
    IMPX977; MeCP2; 蕾特氏癥; RNA-seq

    Keywords:


    Project Supported:
    National Natural Science Foundation of China (No. 81271255)

    Clc Number:

WeChat

Mobile website