醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

Archive > Volume 11 Issue 2 > 2019,11(2):177-184. DOI:10.1016/j.chmed.2018.12.004 Prev Next

加味脈君安片對自發(fā)性高血壓大鼠血壓的影響及其機制研究

Original articles

Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats

  • Article
  • Figures
  • Metrics
  • Preview PDF
  • Reference
  • Related
  • Cited by
  • Materials
    摘要:
    目的：薄膜包衣脈君安片由葛根，氫氯噻嗪，鉤藤（366：1：980）及輔料混合成型，是我國中藥保護品種。本次研究中使用的脈君安片（MJA）由葛根總黃酮，氫氯噻嗪，鉤藤總堿（40:11:75）組成，加味脈君安片（M-MJA）是在脈君安片基礎(chǔ)上加入厚樸總酚構(gòu)成（葛根總黃酮：厚樸總酚：氫氯噻嗪：鉤藤總堿=40：40：11：75）。本次研究主要是探討M-MJA對自發(fā)性高血壓大鼠（SHR）的降壓作用和機制。 方法：將15只SHR隨機分為對照組(MC)、脈君安片給藥組（MJA，180 mg / kg•d）、加味脈君安片給藥組（M-MJA，218 mg / kg•d），每組5只。每2周測血壓 1次，8周后處死動物并檢測與血壓相關(guān)指標。 結(jié)果：結(jié)果表明M-MJA給藥8周后收縮壓與舒張壓明顯低于對照組。M-MJA可降低血漿中腎素，血管緊張素II，醛固酮的水平，降低內(nèi)皮素-1和20-羥基二十碳四烯酸的水平，增加內(nèi)皮性一氧化氮合酶水平，增加Nrf2的蛋白表達和血紅素氧合酶-1，谷胱甘肽過氧化物酶的活性，抑制CYP2E1，CYP 4A的蛋白表達和丙二醛的活性。 結(jié)論：這些結(jié)果表明M-MJA可調(diào)節(jié)腎素 - 血管緊張素 - 醛固酮系統(tǒng)，改善內(nèi)皮功能，抑制CYP4A活性，減少20-HETE的產(chǎn)生，緩解內(nèi)臟器官的氧化應(yīng)激損傷，最終產(chǎn)生抗高血壓的作用。此外，M-MJA調(diào)節(jié)腎素 - 血管緊張素 - 醛固酮系統(tǒng)，改善內(nèi)皮功能，抗氧化能力均比MJA更強，這表明M-MJA可能比MJA具有更好的抗高血壓作用。

    Abstract:
    Objective: Maijunan (MJA) Tablets is a protected variety of traditional Chinese medicine (TCM) consisted of Pueraria lobata, hydrochlorothiazide (HTCZ), Uncaria rhynchophylla (366:1:980) and excipient. In the present work, MJA was consisted of the total flavones of P. lobata, HCTZ and total alkaloids of U. rhynchophylla (40:11:75). The combination of MJA and the total phenols of Magnolia officinalis (M-MJA) was consisted of the total flavones of P. lobata, the total phenols of M. officinalis, HCTZ and the total alkaloids of U. rhynchophylla (40:40:11:75). The aim of this work was to examine the effect and mechanism of M-MJA on the blood pressure of spontaneous hypertensive rats (SHRs). Methods: Adult male SHRs were randomly divided into control group, MJA group (180 mg/kg·d), and the M-MJA group (218 mg/kg·d) (n = 5). SHRs were orally administered with M-MJA and MJA respectively once a day for 8 weeks, the blood pressure of SHRs was measured every two weeks, and the biochemical indicators related to blood pressures were detected at the last dosing. Results: After oral administration of M-MJA to SHRs once a day for 8 weeks, the systolic and diastolic blood pressures of SHRs were deceased significantly. M-MJA affected renin-angiotensin-aldosterone system by decreasing the levels of Ren, Ang II and ALD, affected the endothelial function by decreasing the levels of ET-1 and 20-HETE, and increasing the level of eNOS, affected the oxidative stress by increasing the protein expression of Nrf2 and the activities of HO-1 and GSH-Px, and decreasing the protein expression of CYP2E1 and CYP4A, as well as the content of MDA. Conclusion: These results indicated that M-MJA could regulate the renin-angiotensin-aldosterone system, improve endothelial function, and inhibit CYP4A activity to reduce the production of 20-HETE, alleviate the oxidative stress disorder of the visceral organs, and eventually exert antihypertensive effect. Additionally, the anti-oxidant ability, regulating the renin-angiotensin-aldosterone system and improving endothelial function of M-MJA are more powerful than that of MJA, suggesting that M-MJA may have a better anti-hypertensive effect than MJA.

    關(guān)鍵詞:
    厚樸總酚;脈君安片;內(nèi)皮系統(tǒng);氧化應(yīng)激;腎素 - 血管緊張素 - 醛固酮;自發(fā)性高血壓大鼠

    Keywords:


    Project Supported:
    major technological innovation project of Hubei Province (Grant No. 2016ACA140) and the united fund for innovation and entrepreneurship of Ministry of Education of China (Grant No. 201610512001)

    Clc Number:

WeChat

Mobile website