Metabolic profiling of icaritin in rats using UHPLC-Q/TOF-MS

Tong Wang a; b; 1; Xin-chi Feng a; 1; Li-qin Ding b; Kun Wang a; b; Xu-liu Shi a; b; Li-wei Chai a; b; Yang Li a; b; Feng Qiu a; b*; Tong Wang a; b; 1; Xin-chi Feng a; 1; Li-qin Ding b; Kun Wang a; b; Xu-liu Shi a; b; Li-wei Chai a; b; Yang Li a; b; Feng Qiu a; b*

Archive > Volume 11 Issue 2 > 2019,11(2):185-191. DOI:10.1016/j.chmed.2019.03.008 Prev Next

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Metabolic profiling of icaritin in rats using UHPLC-Q/TOF-MS

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摘要:

Abstract:

Objective: To identify the in vivo metabolites of icaritin and speculate its metabolic profiling in rats. Methods: The plasma, bile, urine, and feces of rats were collected after orally administration of icaritin at a dose of 100 mg/kg and detected by an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS) in both positive and negative modes. The data of treated and control groups were compared and analyzed with the aid of Metabolynx XS software. Results: A total of 25 metabolites were identified in the biosamples, and 14 of them were reported for the first time to our knowledge. Conclusion: The main metabolite types of icaritin in rats were glucuronide conjugation, methylation, hydroxylation, reduction, and acetylation.

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National Natural Science Foundation of China (NSFC) [No. 81430095]

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