醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

Archive > Volume 12 Issue 1 > 2020,12(1):88-94. DOI:https://doi.org/10.1016/j.chmed.2019.11.003 Prev Next

Original articles

A reliable and rapid pharmacokinetic study of pueraria isoflavones using pueraria reference extractive substance in beagle plasma: Application to study of Yufeng Ningxin Tablets

  • Article
  • Figures
  • Metrics
  • Preview PDF
  • Reference
  • Related
  • Cited by
  • Materials
    摘要:


    Abstract:
    Objective: In order to evaluate the reliability and feasibility of pueraria reference extractive substance (RES) used in biological sample, the pharmacokinetics of 3 ? –hydroxy puerarin (3 ? -HP), puerarin, 3 ? – methoxy puerarin (3 ? -MP), and daidzein-8-C-apiosyl-(1-6)-glucoside (DAG) in beagle plasma following oral administration of Yufeng Ningxin Tablet were quantitated. Methods: A reliable and sensitive high-performance liquid chromatography-tandem triple quadrupole mass spectrometry (HPLC-QQQ-MS/MS) method developed with chromatographic separation was oper- ated on a Merck C 18 column, and acetonitrile-5 mmol/L ammonium was used as mobile phase in gradient elution. The plasma samples were deproteinized by acetone, detected by triple quadrupole mass spec- trometry with an electrospray ionization interface, and quantified using selected ion monitoring mode. The pharmacokinetic parameters were calculated by Winnonlin 4.1. Results: The calibration curves of the reference extractive substance and standard substance methods were linear over the ranges 0.0417–11.3309 μg/mL and 0.0394–10.0000 μg/mL. The intra-day and inter- day precision of the two methods at three concentrations were less than 13.63%, and the average recov- eries of 3 ? -HP, puerarin, 3 ? -MP, and DAG were more than 70.67%. The RSD of the mean plasma concentra- tions of the analytes calculated by the two methods was less than 5%, and cos (?) = =1.000. Among the analytes, puerarin showed the highest blood concentration [(940 ± 185) ng/mL] and the longest retention time [(5 ± 1) h] in the dog’s bodies. Conclusion: Pueraria reference extractive substance can be seen as an alternative to the standard sub- stance to overcome the scarcity of standard substance for the analysis of biological samples.

    關(guān)鍵詞:


    Keywords:


    Project Supported:
    We are grateful for financial support from the Major or Scien- tific and Technological Special Project for “Significant New Drug Creation” (No. 2014ZX0930437), the support of Conservation Prop- agation and Quality Standard of Characteristic Advantage of Chi- nese Herbal Medicine(No. 2015BAI05B03), Bijie City Achievement Transformation Project (Bi Ke Cheng Zi [2014] No. 8), Bijie City So- cial Development Project (Bi Kehe Zi [2015] No. 41).

    Clc Number:

WeChat

Mobile website