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Archive > Volume 15 Issue 1 > 2023,15(1):57-62. DOI:https://doi.org/10.1016/j.chmed.2022.03.006 Prev Next

生長素依賴血紅素加氧酶誘導(dǎo)柴胡側(cè)根生成

Original articles

Auxin induces lateral root formation in Bupleurum: A heme oxygenase dependent approach

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    摘要:
    目的：柴胡屬植物的柴胡皂苷含量隨側(cè)根數(shù)量的增加而增加，但其遺傳機制尚不清楚。本研究旨在鑒定北柴胡（B. chinense）和狹葉柴胡（B. scorzonerifolium）的血紅素加氧酶（HO）基因家族成員，并評估它們在柴胡根系發(fā)育中的作用。 研究方法：從北柴胡和狹葉柴胡的全長轉(zhuǎn)錄組數(shù)據(jù)中篩選出HO家族的基因序列，并對其理化性質(zhì)、保守結(jié)構(gòu)域、結(jié)構(gòu)式和系統(tǒng)發(fā)育關(guān)系進行分析。此外，還通過轉(zhuǎn)錄組測序和qRT-PCR比較了HO基因在兩種植物根系不同部位的表達模式。 結(jié)果：從轉(zhuǎn)錄組數(shù)據(jù)中確定了屬于 HO1亞家族的五個柴胡HO基因（BcHO1-BcHO5），HO2亞家族成員未被確定。在轉(zhuǎn)錄組分析中，BcHO1和BcHO2的表達水平明顯高于其他三個HO成員。此外，BcHO1的表達譜與B. chinense和B. scorzonerifolium的側(cè)根發(fā)育一致。 結(jié)論： 血紅素加氧酶（HO）可能參與了生長素誘導(dǎo)側(cè)根形態(tài)發(fā)生。通過調(diào)節(jié)這些基因的表達可提高柴胡皂苷的產(chǎn)量。

    Abstract:
    Objective: The content of saikosaponins in genus Bupleurum is increased with numbers of lateral root, but the genetic mechanisms are largely unknown. This study aims to identify the heme oxygenase (HO) gene family members of B. chinense and B. scorzonerifolium, and assess their role in the root development in Bupleurum. Methods: The gene sequences of HO family were selected from iso-seq full-length transcriptome data of B. chinense and B. scorzonerifolium, and were analyzed in physicochemical properties, conserved domains, motifs and phylogenetic relationship. In addition, the expression patterns of HO gene in different parts of roots were compared via transcriptome sequencing and qRT-PCR in the two species. Results: Five Bupleurum HO genes (BcHO1-BcHO5) belonging to the HO1 subfamily were identified from the transcriptome data, whereas the HO2 subfamily member was not identified. The expression levels of BcHO1 and BcHO2 were significantly higher than those of other three HO members in the transcriptome analysis. In addition, the expression profile of BcHO1 showed consistency with lateral root development in B. chinense and B. scorzonerifolium. Conclusion: Hos might participate in the auxin-induced morphogenesis of lateral roots. The yield of saikosaponin may be improved by manipulating expression of these genes.

    關(guān)鍵詞:
    生長素;柴胡屬;血紅素加氧酶;側(cè)根形成

    Keywords:


    Project Supported:
    This work was financially supported by China Agriculture Research System of MOF and MARA, the CAMS Innovation Fund for Medical Sciences (CIFMS) (No. 2016-I2M-2-003), Opening Project Fund of Key Laboratory of Rubber Biology and Genetic Resource Utilization, Ministry of Agriculture/State Key Laboratory Breeding Base of Cultivation & Physiology for Tropical Crops/Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture” (No. RRL-KLOF202201).

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