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Archive > Volume 15 Issue 1 > 2023,15(1):102-109. DOI:https://doi.org/10.1016/j.chmed.2022.03.008 Prev Next

肉蓯蓉對(duì)慶大霉素誘導(dǎo)腎損傷大鼠的腎保護(hù)作用

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Protective effect of Cistanche deserticola on gentamicin-induced nephrotoxicity in rats

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    摘要:
    目的：慶大霉素是一種被廣泛使用的氨基糖苷類(lèi)抗生素，但是其腎毒性限制了其使用。本研究的主要目的就是評(píng)價(jià)肉蓯蓉水提取物（CDW）和75%乙醇提物（CDE）對(duì)慶大霉素誘導(dǎo)的腎損傷大鼠的緩解作用。 方法：慶大霉素（100mg/kg）通過(guò)腹腔注射給藥連續(xù)10天誘導(dǎo)大鼠腎損傷模型。腎小球?yàn)V過(guò)率、血清特異性腎功能參數(shù)（尿素氮和肌酐）和腎臟組織病理切片被用來(lái)評(píng)價(jià)慶大霉素誘導(dǎo)的腎毒性。腎臟氧化應(yīng)激（CAT、SOD、GSH和MDA）、炎癥（MPO、TNF-α、IL-6和NF-κB）和凋亡（Bax、Bcl-2）標(biāo)記物也被檢測(cè)。 結(jié)果： 結(jié)果表明，慶大霉素水提取物和75%乙醇提取物（分別命名為CDW和CDE）(100、200、400 mg/kg)與GM聯(lián)用，可恢復(fù)GM引起的腎小球?yàn)V過(guò)率下降，增強(qiáng)腎臟內(nèi)源性抗氧化能力。CDW或CDE組GM誘導(dǎo)的腎炎性細(xì)胞因子（腫瘤壞死因子α和白細(xì)胞介素6)、核因子κB核蛋白(P65)表達(dá)增加，髓過(guò)氧化物酶活性降低。CDW或CDE均能顯著降低GM腎毒性大鼠Bax蛋白表達(dá)，增加Bcl-2蛋白表達(dá)。 結(jié)論：本研究的結(jié)果表明肉蓯蓉可以通過(guò)減少腎臟的氧化應(yīng)激，炎癥和細(xì)胞凋亡來(lái)減輕慶大霉素誘導(dǎo)的腎臟功能障礙和組織損傷。

    Abstract:
    Objective: Gentamicin (GM) is a commonly used aminoglycoside antibiotic, however, renal toxicity has limited its usage. The present study was designed to evaluate the ameliorative effect of Cistanche deserticola on GM-induced nephrotoxicity in rats. Methods: The nephrotoxicity in rats was induced by intraperitoneal administration of GM (100 mg/kg) for 10 consecutive days. Glomerular filtration rate, blood urea nitrogen, creatinine and kidney histopathology were detected to assess the GM-induced nephrotoxicity. The oxidative stress (catalase, superoxide dismutase, glutathione and malondialdehyde) was assessed. The inflammatory response (tumor necrosis factor-a, interleukin-6, myeloperoxidase and nuclear factor-kappa B) and apoptotic marker (Bax and Bcl-2) were also evaluated. Results: The results showed that water and 75% ethanol extracts of C. deserticola (named CDW and CDE, respectively) (100, 200 and 400 mg/kg) in combination with GM could recover the reduction of glomerular filtration rate and enhance the renal endogenous antioxidant capability induced by GM. The increase in the expression of renal inflammatory cytokines (tumor necrosis factor-a and interleukin-6), nuclear protein of nuclear factor-kappa B (p65) and the activity of myeloperoxidase induced by GM was significantly decreased upon CDW or CDE treatment. In addition, CDW or CDE treatment could decrease the Bax protein expression and increase the Bcl-2 protein expression in GM-induced nephrotoxicity in rats significantly. Conclusion: The study demonstrated that C. deserticola treatment could attenuate kidney dysfunction and structural damage in rats induced by GM through the reduction of inflammation, oxidative stress and apoptosis.

    關(guān)鍵詞:
    凋亡;肉蓯蓉;慶大霉素;炎癥;腎毒性;列當(dāng)科;氧化應(yīng)激

    Keywords:


    Project Supported:
    This work was supported by the National Key Research and Development Program of China (2018YFC1706300, 2018YFC17063003), the Key Research and Development Project of GanSu Province (20YF2FA019), the People’s Livelihood Special Project from Technical Innovation Guide Plan of Gansu Province (20CX4FK014), the Key Talent Project of Organization Department of Gansu Provincial Party Committee ([2022] Gan-71), the Talent Innovation and Entrepreneurship Project of Lanzhou (2017-RC115, 2020-RC-41).

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