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Archive > Volume 15 Issue 1 > 2023,15(1):132-138. DOI:https://doi.org/10.1016/j.chmed.2022.09.003 Prev Next

α-紫羅蘭酮對UVB誘導(dǎo)的表皮角質(zhì)形成細胞光老化具有保護作用

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α-Ionone protects against UVB-induced photoaging in epidermal keratinocytes

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    摘要:
    目的：評價α-紫羅蘭酮，一種主要存在于樹莓、胡蘿卜、烤杏仁、水果和草藥中的芳香化合物，是否能抑制UVB介導(dǎo)的人表皮角化細胞系(HaCaT細胞)的光老化和屏障功能障礙。 方法：通過檢測HaCaT細胞中屏障相關(guān)基因和基質(zhì)金屬蛋白酶(matrix metalloproteinases，MMPs)的表達，評價α-紫羅蘭酮的抗光老化作用，進一步分析氧化產(chǎn)物、活性氧、抗氧化酶和炎癥因子水平，以闡明α-紫羅蘭酮對表皮光老化的保護作用。 結(jié)果：α-紫羅蘭酮可逆轉(zhuǎn)HaCaT細胞中角蛋白1和聚絲蛋白的表達，減弱UVB誘導(dǎo)的屏障功能障礙。α-紫羅蘭酮還能降低UVB照射的HaCaT細胞中MMP-1的蛋白含量以及MMP-1和MMP-3的mRNA的表達量，提示其對細胞外基質(zhì)具有保護作用。此外，與UVB照射的HaCaT細胞相比，α-紫羅蘭酮處理的HaCaT細胞中白細胞介素(interleukin，IL)-1β、IL-6、IL-8和腫瘤壞死因子-α顯著降低。α-紫羅蘭酮處理顯著抑制UVB誘導(dǎo)的細胞內(nèi)活性氧增加和丙二醛積累。因此，α-紫羅蘭酮抑制MMP分泌和屏障損傷的有益作用可能與減輕炎癥和氧化應(yīng)激有關(guān)。 結(jié)論：我們的結(jié)果強調(diào)了α-紫羅蘭酮對表皮光老化的保護作用并促進其作為一種潛在的天然抗光損傷劑的臨床應(yīng)用。

    Abstract:
    Objective: To evaluate whether α-ionone, an aromatic compound mainly found in raspberries, carrots, roasted almonds, fruits, and herbs, inhibits UVB-mediated photoaging and barrier dysfunction in a human epidermal keratinocyte cell line (HaCaT cells). Methods: The anti-photoaging effect of α-ionone was evaluated by detecting the expression of barrier-related genes and matrix metalloproteinases (MMPs) in HaCaT cells. The levels of reactive oxygen species, oxidation product, antioxidant enzyme, and inflammatory factors were further analysed to underline the protective effect of α-ionone on epidermal photoaging. Results: It was found that α-ionone attenuated UVB-induced barrier dysfunction by reversing keratin 1 and filaggrin in HaCaT cells. α-Ionone also reduced the protein amount of MMP-1 and mRNA expression of MMP-1 and MMP-3 in UVB-irradiated HaCaT cells, implying protective effects on extracellular matrix. Furthermore, HaCaT cells exposed to a-ionone showed significant decreases in interleukin (IL)-1b, IL-6, IL-8, and tumor necrosis factor-a as compared to UVB-irradiated HaCaT cells. a-Ionone treatment significantly inhibited the UVB-induced intracellular reactive oxygen species increase and malondialdehyde accumulation. Therefore, the beneficial effects of a-ionone on inhibiting MMPs secretion and barrier damage may be related to attenuated inflammation and oxidative stress. Conclusion: Our results highlight the protective effects of α-ionone on epidermal photoaging and promote its clinic application as a potential natural anti-photodamage agent in future.

    關(guān)鍵詞:
    α-紫羅蘭酮;表皮角質(zhì)形成細胞;光老化;UVB

    Keywords:


    Project Supported:
    This work is supported by Shandong Provincial Natural Science Foundation (ZR2021QC118), Beijing Natural Science Foundation (7222249), and the 2115 Talent Development Program of China Agricultural University.

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