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Archive > Volume 15 Issue 1 > 2023,15(1):139-150. DOI:https://doi.org/10.1016/j.chmed.2022.08.002 Prev Next

UPLC-Q-TOF/MS法鑒定血府逐瘀湯灌胃大鼠血清中的原型成分及其代謝產(chǎn)物

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Identification of prototype compounds and their metabolites in rats’ serum from Xuefu Zhuyu Decoction by UPLC-Q-TOF/MS

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    摘要:
    目的：血府逐瘀湯作為經(jīng)典方劑，廣泛應(yīng)用于心腦血管疾病的治療。為了系統(tǒng)揭示潛在的有效成分，我們建立了一種快速的超高效液相色譜-四極桿飛行時(shí)間質(zhì)譜（UPLC-Q-TOF/MS）法，以期鑒定血府逐瘀湯灌胃大鼠血清中的原型成分及其代謝產(chǎn)物。方法：采用UPLC-Q-TOF/MS法分析血府逐瘀湯灌胃大鼠血清中的原型成分及其代謝產(chǎn)物，通過保留時(shí)間、質(zhì)譜數(shù)據(jù)、特征裂解模式及文獻(xiàn)推測(cè)了血清中的原型成分及其代謝產(chǎn)物，并通過與對(duì)照品比對(duì)進(jìn)一步鑒定。結(jié)果：共鑒定了175個(gè)成分（24個(gè)原型成分和151個(gè)代謝物），其主要代謝途徑包括葡萄糖醛酸化反應(yīng)、水解反應(yīng)、硫酸化反應(yīng)、去甲基化反應(yīng)和羥基化反應(yīng)等。結(jié)論：本研究建立了一種UPLC-Q-TOF/MS方法，用于分析血府逐瘀湯灌胃大鼠血清中的原型成分及其代謝產(chǎn)物，為進(jìn)一步闡明血府逐瘀湯的有效成分提供了依據(jù)。

    Abstract:
    Objective: As a classic prescription in traditional Chinese medicine, Xuefu Zhuyu Decoction (XFZYD) has been widely used in the clinical treatment of cardiovascular and cerebrovascular diseases. In order to unveil the potentially effective compounds, a rapid ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) method was established to identify prototype compounds and their metabolites from XFZYD in rats’ serum. Methods: The serum from rats after intragastric administration of XFZYD aqueous extract was analyzed by UPLC-Q-TOF/MS method. The prototype compounds and their metabolites were identified by comparison with the reference standards and tentatively characterized by comprehensively analyzing the retention time, MS data, characteristic MS fragmentation pattern and retrieving literatures. Results: A total of 175 compounds (24 prototype compounds and 151 metabolites) were identified and tentatively characterized. The metabolic pathways of prototype compounds in vivo were also summarized, including glucuronidation, hydrolyzation, sulfation, demethylation, and hydroxylation, and so on. Conclusion: In this study, a UPLC-Q-TOF/MS technique was developed to analyze prototype compounds and their metabolites from XFZYD in serum, which would provide the evidence for further studying the effective compounds of XFZYD.

    關(guān)鍵詞:
    代謝產(chǎn)物;原型成分;血清;UPLC-Q-TOF/MS;血府逐瘀湯

    Keywords:


    Project Supported:
    This work was supported by National Natural Science Foundation of China (No. 81873192 and 81202877) and Postgraduate Research and Innovation Project of Tianjin (No. 2019YJSS197).

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