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Archive > Volume 15 Issue 2 > 2023,15(2):310-316. DOI:https://doi.org/10.1016/j.chmed.2022.06.013 Prev Next

轉(zhuǎn)錄組和代謝組聯(lián)合分析肉桂不同部位的有效成分差異的分子機(jī)制

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De novo transcriptome assembly and metabolomic analysis of three tissue types in Cinnamomum cassia

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    摘要:
    目的：肉桂的樹皮、葉子和樹枝在歷史上一直被用作中藥、香料和食品防腐劑，其中苯丙類化合物是主要的化合物。然而，肉桂中的苯丙類生物合成途徑尚不清楚。我們通過對與苯丙烷類生物合成相關(guān)的差異表達(dá)基因的描述性分析來闡明這些途徑，并鑒定各種苯丙烷類代謝產(chǎn)物。 方法：對肉桂不同部位進(jìn)行化學(xué)分析、代謝組測序和轉(zhuǎn)錄組測序，以研究樹皮、枝和葉中活性成分含量差異的分子機(jī)制。 結(jié)果：代謝組學(xué)分析顯示，少量黃酮類化合物、香豆素和肉桂醛在葉和枝中積累。轉(zhuǎn)錄組分析表明，相對于樹皮，與苯丙烷類和類黃酮生物合成相關(guān)的基因在葉和枝中被下調(diào)。觀察到的三種部位中精油含量的差異可能歸因于苯丙烷類和黃酮類代謝途徑中涉及的基因的差異表達(dá)。 結(jié)論：本研究通過比較轉(zhuǎn)錄組和代謝組，確定了苯丙烷類途徑中控制樹皮、枝葉中黃酮、香豆素和肉桂醛含量的關(guān)鍵基因。這些發(fā)現(xiàn)可能有助于評估苯丙烷類和黃酮類代謝產(chǎn)物，并鑒定與肉桂中苯丙烷類化合物和黃酮類化合物合成相關(guān)的特定候選基因。

    Abstract:
    Objective: The barks, leaves, and branches of Cinnamomum cassia have been historically used as a traditional Chinese medicine, spice, and food preservative, in which phenylpropanoids are responsible compounds. However phenylpropanoid biosynthesis pathways are not clear in C. cassia. We elucidated the pathways by descriptive analyses of differentially expressed genes related to phenylpropanoid biosynthesis as well as to identify various phenylpropanoid metabolites. Methods: Chemical analysis, metabolome sequencing, and transcriptome sequencing were performed to investigate the molecular mechanisms underlying the difference of active components content in the barks, branches and leaves of C. cassia. Results: Metabolomic analysis revealed that small amounts of flavonoids, coumarine, and cinnamaldehyde accumulated in both leaves and branches. Transcriptome analysis showed that genes associated with phenylpropanoid and flavonoid biosynthesis were downregulated in the leaves and branches relative to the barks. The observed differences in essential oil content among the three tissues may be attributable to the differential expression of genes involved in the phenylpropanoid and flavonoid metabolic pathways. Conclusion: This study identified the key genes in the phenylpropanoid pathway controling the flavonoid, coumarine, and cinnamaldehyde contents in the barks, branches and leaves by comparing the transcriptome and metabolome. These findings may be valuable in assessing phenylpropanoid and flavonoid metabolites and identifying specific candidate genes that are related to the synthesis of phenylpropanoids and flavonoids in C. cassia.

    關(guān)鍵詞:
    肉桂;代謝組;苯丙烷類生物合成;轉(zhuǎn)錄組

    Keywords:


    Project Supported:
    The Guangdong Basic and Applied Basic Research Foundation (No. 2019A1515111043), Basic Research Project of Luoding Cinnamon Industry Development (No. 2018-082), Youth Innovative Talents Project by Educational Department of Guangdong Province (No. 2019KQNCX057), Special Fund for Science and Technology Innovation Cultivation of Guangdong University Students (special fund for ‘‘climbing plan”) (No. 51348227), and the Scientific Research Project of the Traditional Chinese Medicine Bureau of Guangdong Province (No. 20202104) supported this study.

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