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Archive > Volume 15 Issue 2 > 2023,15(2):317-328. DOI:https://doi.org/10.1016/j.chmed.2022.06.009 Prev Next

基于UPLC-Q-Exactive-MS/MS 和 HPLC法對(duì)假東風(fēng)草和東風(fēng)草的化學(xué)成分分析和快速鑒別

Original articles

Chemical profiling and rapid discrimination of Blumea riparia and Blumea megacephala by UPLC-Q-Exactive-MS/MS and HPLC

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    摘要:
    目的：快速鑒定兩種原植物形態(tài)和化學(xué)成分都非常相似的中藥材假東風(fēng)草和東風(fēng)草，并研究二者的化學(xué)成分差異。 方法：利用UPLC-Q-Exactive-MS/MS分析和鑒定假東風(fēng)草和東風(fēng)草中的化學(xué)成分，進(jìn)一步通過化學(xué)模式識(shí)別分析（CPR）比較和鑒別兩種中藥材，并確定其潛在的標(biāo)志性成分。同時(shí)，建立了一種HPLC方法評(píng)價(jià)兩種中藥材的質(zhì)量。 結(jié)果：從兩種藥材中共鑒定了93種成分，其中包括54種酚酸類成分，35種黃酮類成分，2種糖類成分，1種酚酸苷和1種其他成分，其中67種是在假東風(fēng)草和東風(fēng)草中首次鑒定。化學(xué)模式識(shí)別分析結(jié)果表明，根據(jù)LC-MS數(shù)據(jù)，可以明顯區(qū)分假東風(fēng)草和東風(fēng)草。利用HPLC法得到的峰面積，計(jì)算異綠原酸A與隱綠原酸的峰面積比值可以區(qū)分假東風(fēng)草和東風(fēng)草，并對(duì)其藥材質(zhì)量進(jìn)行控制。 結(jié)論：假東風(fēng)草和東風(fēng)草在化學(xué)成分方面存在顯著差異。本實(shí)驗(yàn)為比較和評(píng)價(jià)假東風(fēng)草和東風(fēng)草的質(zhì)量提供了一種快速、便捷的方法。

    Abstract:
    Objective: To rapidly identify the two morphologies and chemical properties of similar herbal medicines, Blumea riparia and B. megacephala as the basis for chemical constituent analysis. Methods: UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in B. riparia and B. megacephala. Chemical pattern recognition (CPR) was further used to compare and distinguish the two herbs and to identify their potential characteristic markers. Then, an HPLC method was established for quality evaluation. Results: A total of 93 constituents are identified, including 54 phenolic acids, 35 flavonoids, two saccharides, one phenolic acid glycoside, and one other constituent, of which 67 were identified in B. riparia and B. megacephala for the first time. CPR indicates that B. riparia and B. megacephala samples can be distinguished from each other based on the LC–MS data. The isochlorogenic acid A to cryptochlorogenic acid peak area ratio calculated from the HPLC chromatograms was proposed as a differentiation index for distinguishing and quality control of B. riparia and B. megacephala. Conclusion: This study demonstrates significant differences between B. riparia and B. megacephala in terms of chemical composition. The results provide a rapid and simple strategy for the comparison and evaluation of the quality of B. riparia and B. megacephala.

    關(guān)鍵詞:
    假東風(fēng)草;東風(fēng)草;化學(xué)模式識(shí)別;UPLC-Q-Exactive-MS/MS

    Keywords:


    Project Supported:
    We are grateful to Yanan Cao for her technical support of the UPLC-Q-Exactive-MS/MS. This study was supported by National Key Research and Development Program of China (No. 2018YFC1706101), The Sichuan Key Research and Development Program (No. 2021YFS0043), Leading Talent Support Program of National Civil Commission (2021), and Special Fund of Natural Science for the Central Universities (No. 2020NQN42).

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