0.9993) in a wide concentration range. Their average recoveries were 99.50%, 95.38%, 97.75%, 96.00%, 98.20%, 97.50%, 95.50%, 99.33%, 96.75%, 96.50%, 98.50%, 97.83%, 99.20%, 95.33%, 97.33% and 96.30%, respectively, and the RSD were 1.99%, 1.81%, 1.63%, 1.98%, 1.67%, 1.92%, 1.74%, 1.67%, 1.90%, 1.72%, 1.88%, 1.83%, 1.79%, 1.76%, 1.81% and 1.96%, respectively. Conclusion: Based on the results of the HPLC analysis, it was concluded that p-hydroxycinnamic acid (1), O-hydroxycinnamic acid (2), coniferyl alcohol (5), 5,4′-dihydroxy-7,3′-dimethoxyflavanone (8), 5,4′-dihy droxy-7-methoxyflavanone (9), 5-hydroxy-7,4′-dimethoxyflavanone (12), dehydrofalcarindiol (13), arteordoyn A (14), dehydrofalcarinol (15) and capillarin (16) are best suited for the role of quality indicators of A. ordosica grown in different ecological environments."/>

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Archive > Volume 15 Issue 2 > 2023,15(2):337-342. DOI:https://doi.org/10.1016/j.chmed.2022.08.009 Prev Next

黑沙蒿中16個化合物的HPLC分析

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HPLC analysis of 16 compounds from Artemisia ordosica

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    摘要:
    目的：建立同時測定黑沙蒿中16個化合物含量的高效液相色譜法。 方法：采用高效液相色譜法對黑沙蒿藥材的16個指標成分進行分析。高效液相色譜條件為：Agilent Eclipse Plus C18色譜柱(250 mm × 4.6 mm, 5 μm)，乙腈(A)-水(B)為流動相，梯度洗脫：0 ~ 10 min，75% ~ 65% B；10 ~ 30 min，65% ~ 35% B；流速為1.0 mL/min，柱溫為40℃，進樣量為10 μL，化合物1 ~ 10、12在285 nm處檢測，化合物11、13 ~ 16在225 nm處檢測。 結(jié)果：在所選擇的色譜條件下，化合物1 ~ 16在較寬的濃度范圍內(nèi)呈良好的線性關(guān)系(r > 0.9993)。它們的平均回收率分別為99.50%, 95.38%,97.75%,96.00%,98.20%,97.50%,95.50%,99.33%,96.75%,96.50%,98.50%,97.83%,99.20%,95.33%,97.33%和96.30%, RSD分別為1.99%,1.81%,1.63%,1.98%,1.67%,1.92%,1.74%,1.67%,1.90%,1.72%,1.88%,1.83%,1.79%,1.76%,分別為1.81%和1.96%。 結(jié)論：根據(jù)HPLC分析結(jié)果，對羥基肉桂酸(1)、鄰羥基肉桂酸(2)、松柏醇(5)、5,4'-二羥基-7,3'-二甲氧基黃酮(8)、5,4'-二羥基-7-甲氧基黃酮(9)、5-羥基-7,4'-二甲氧基黃酮(12)、dehydrofalcarindiol (13)、arteordoyn A (14)、dehydrofalcarinol (15)和capillarin (16)最適合作為不同生態(tài)環(huán)境下的黑沙蒿質(zhì)量評價指標。

    Abstract:
    Objective: To establish a high-performance liquid chromatographic method (HPLC) for the simultaneous determination of 16 compounds from Artemisia ordosica. Methods: HPLC was used to analyze 16 quality indicators of A. ordosica. The HPLC conditions were as follows: Agilent Eclipse Plus C18 column (250 mm × 4.6 mm, 5 lm) with acetonitrile (A)-water (B) as mobile phase, gradient elution: 0–10 min, 75%–65% B; 10–30 min, 65%–35% B; and finally 30–40 min, 35%–15% B. The flow rate was 1.0 mL/min, the column temperature was 40 ℃, the injection volume was 10 μL, and monitored by absorbance at 285 nm for compounds 1–10, 12 and 225 nm for compounds 11, 13–16. Results: Under the selected experimental chromatographic conditions, compounds 1–16 showed good linearity (r > 0.9993) in a wide concentration range. Their average recoveries were 99.50%, 95.38%, 97.75%, 96.00%, 98.20%, 97.50%, 95.50%, 99.33%, 96.75%, 96.50%, 98.50%, 97.83%, 99.20%, 95.33%, 97.33% and 96.30%, respectively, and the RSD were 1.99%, 1.81%, 1.63%, 1.98%, 1.67%, 1.92%, 1.74%, 1.67%, 1.90%, 1.72%, 1.88%, 1.83%, 1.79%, 1.76%, 1.81% and 1.96%, respectively. Conclusion: Based on the results of the HPLC analysis, it was concluded that p-hydroxycinnamic acid (1), O-hydroxycinnamic acid (2), coniferyl alcohol (5), 5,4′-dihydroxy-7,3′-dimethoxyflavanone (8), 5,4′-dihy droxy-7-methoxyflavanone (9), 5-hydroxy-7,4′-dimethoxyflavanone (12), dehydrofalcarindiol (13), arteordoyn A (14), dehydrofalcarinol (15) and capillarin (16) are best suited for the role of quality indicators of A. ordosica grown in different ecological environments.

    關(guān)鍵詞:
    黑沙蒿;HPLC;質(zhì)量指標;同時測定

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    Project Supported:
    This work was supported by the National Key R&D Program of China (2019YFC1712300).

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