An optimal medicinal and edible Chinese herbal formula attenuates particulate matter-induced lung injury through its anti-oxidative, anti-inflammatory and anti-apoptosis activities

doi:https://doi.org/10.1016/j.chmed.2022.08.003

Archive > Volume 15 Issue 3 > 2023,15(3):407-420. DOI:https://doi.org/10.1016/j.chmed.2022.08.003 Prev Next

一種最佳的藥用和食用中藥配方，通過其抗氧化、抗炎和抗細(xì)胞凋亡的活性來減輕可吸入顆粒物引起的肺損傷

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An optimal medicinal and edible Chinese herbal formula attenuates particulate matter-induced lung injury through its anti-oxidative, anti-inflammatory and anti-apoptosis activities

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摘要:

目的：確定預(yù)防顆粒物(PM)誘導(dǎo)的肺損傷的新策略對于降低慢性呼吸系統(tǒng)疾病的發(fā)病率至關(guān)重要。以中藥方劑為代表的聯(lián)合干預(yù)，同時針對多種病理過程，比單一干預(yù)更有利。本文旨在設(shè)計(jì)一種安全有效的抗PM致肺損傷中草藥配方。方法：采用PM誘導(dǎo)的氧化應(yīng)激、炎癥反應(yīng)和凋亡A549細(xì)胞模型分別篩選抗氧化、抗炎和抗凋亡的MECHs。采用網(wǎng)絡(luò)藥理學(xué)方法，合理設(shè)計(jì)新型中藥配方。采用超高效液相色譜-質(zhì)譜聯(lián)用技術(shù)對MECHs配方進(jìn)行質(zhì)量控制。測定了MECHs配方磁性氧化鐵納米球在斑馬魚體內(nèi)的排泄量。通過小鼠實(shí)驗(yàn)研究了MECH方對PM致肺損傷的作用。結(jié)果：選取五種藥材，分別為橘紅、白扁豆、白術(shù)、桑葉、玉竹，進(jìn)行合理設(shè)計(jì)，形成新的MECH配方。該配方能有效促進(jìn)磁性氧化鐵納米球在斑馬魚體內(nèi)的排泄。中、高劑量方通過提高SOD和GSH-Px活性，降低MDA和ROS水平，降低促炎細(xì)胞因子(IL-6、IL-8、IL-1β和TNF-α)上調(diào)，下調(diào)NF-κB、STAT3和Caspase-3蛋白表達(dá)，顯著預(yù)防PM誘導(dǎo)的小鼠肺損傷。結(jié)論：本研究結(jié)果表明，有效的MECHs配方將成為預(yù)防PM誘導(dǎo)肺損傷的新策略，并為MECHs功能食品的開發(fā)提供范例。

Abstract:

Objective: Identifying novel strategies to prevent particulate matter (PM)-induced lung injury is crucial for the reduction of the morbidity of chronic respiratory diseases. The combined intervention represented by herbal formulae for simultaneously targeting multiple pathological processes can provide a more beneficial effect than the single intervention. The aim of this paper is therefore to design a safe and effective medicinal and edible Chinese herbs (MECHs) formula against PM-induced lung injury. Methods: PM-induced oxidative stress, inflammatory response and apoptosis A549 cell model were used to screen anti-oxidant, anti-inflammatory and anti-apoptotic MECHs, respectively. A network pharmacology method was utilized to rationally design a novel herbal formula. Ultra performance liquid chromatography-mass spectrometer was utilized to assess the quality control of MECHs formula. The excretion of magnetic iron oxide nanospheres of the MECHs formula was estimated in zebrafish. The MECH formula against PM-induced lung injury was investigated with mice experiments. Results: Five selected herbs were rationally designed to form a new MECH formula, including Citri Exocarpium Rubrum (Juhong), Lablab Semen Album (Baibiandou), Atractylodis Macrocephalae Rhizoma (Baizhu), Mori Folium (Sangye) and Polygonati Odorati Rhizoma (Yuzhu). The formula effectively promoted the magnetic iron oxide nanospheres excretion in zebrafish. The mid/high dose formula significantly prevented PM-induced lung damage in mice by enhancing the activity of SOD and GSH-Px, reducing the MDA and ROS level and attenuating the upregulation of pro-inflammatory cytokine (IL-6, IL-8, IL-1β and TNF-α), down regulating the protein expression of NF-κB, STAT3 and Caspase-3. Conclusion: Our findings suggest that the effective MECHs formula will become a novel strategy for preventing PM-induced lung injury and provide a paradigm for the development of functional foods using MECHs.

關(guān)鍵詞:

細(xì)胞凋亡;炎癥反應(yīng);肺損傷;中草藥和食用草藥;氧化應(yīng)激;可吸入顆粒物

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This research was funded by China Postdoctoral Science Foundation (2020T130093ZX).

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