1 and a P-value ＜ 0.05 associated with the separation of different origins were identified. Conclusion: HPLC fingerprint was established to evaluate the quality and authenticity of P. heterophylla. The present work showed that the difference of geographic distributions had an influence on the internal chemical compositions. A sensitive and rapid untargeted metabolomics approach by UHPLC-Q Exactive HF orbitrap MS was utilized to evaluate P. heterophylla from different origins in China for the first time. Overall, this study provides insights to metabolomics of P. heterophylla and supplies important reference values for the development of functional foods."/>

醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

Archive > Volume 15 Issue 3 > 2023,15(3):439-446. DOI:https://doi.org/10.1016/j.chmed.2022.10.005 Prev Next

不同產(chǎn)地太子參的化學(xué)特征比較研究

Original articles

Comparative chemical characters of Pseudostellaria heterophylla from geographical origins of China

  • Article
  • Figures
  • Metrics
  • Preview PDF
  • Reference
  • Related
  • Cited by
  • Materials
    摘要:
    目的:太子參是近年來(lái)備受關(guān)注的一種藥食同源的中藥材?！吨袊?guó)藥典》（2020版)中對(duì)太子參的含量測(cè)定未作規(guī)定。不同產(chǎn)地的環(huán)境條件對(duì)太子參品質(zhì)有一定的影響。本研究的目的是對(duì)中國(guó)不同產(chǎn)地的太子參進(jìn)行鑒別。 方法:采用苯酚-硫酸法測(cè)定28批太子參多糖的含量。采用優(yōu)化的HPLC-PDA法建立指紋圖譜。隨后，對(duì)太子參環(huán)肽B進(jìn)行了相似度分析和定量分析。采用UHPLC- Q Exactive HF orbitrap MS對(duì)太子參代謝產(chǎn)物進(jìn)行了鑒定和評(píng)價(jià)。基于峰面積進(jìn)行主成分分析(PCA)、偏最小二乘判別分析(PLS-DA)、聚類分析(HCA)和正交偏最小二乘判別分析(OPLS-DA)。 結(jié)果:貴州、江蘇兩地藥材多糖含量均高于其他產(chǎn)地，且不同產(chǎn)地藥材多糖含量差異顯著。安徽和江蘇的環(huán)肽B含量較高。28批樣品的HPLC指紋圖譜相似度在0.877 ~ 0.990范圍內(nèi)，可用于鑒別和評(píng)價(jià)太子參藥材質(zhì)量。采用PCA、PLS-DA、HCA、OPLS-DA等多元統(tǒng)計(jì)分析方法對(duì)福建、貴州、江蘇三省的樣品進(jìn)行了分析，表明其代謝成分存在顯著差異。最終，通過(guò)VIP值> 1和P值< 0.05篩選出15種差異性代謝物。 結(jié)論:建立了鑒別太子參質(zhì)量和真實(shí)性的HPLC指紋圖譜。研究表明，地理分布的差異對(duì)其內(nèi)部化學(xué)成分有一定的影響。本文首次采用UHPLC-Q Exactive HF orbitrap MS方法對(duì)中國(guó)不同產(chǎn)地的太子參進(jìn)行了快速、靈敏的非靶向代謝組學(xué)研究。本研究為太子參代謝組學(xué)研究提供了新的思路，為功能性食品的開發(fā)提供了重要的參考價(jià)值。

    Abstract:
    Objective: Pseudostellaria heterophylla has been paid more attention in recent years, mainly as a medicine food homology plant. The content determination of P. heterophylla is not specified in the Chinese Pharmacopoeia (version 2020). The environmental conditions in different production areas could exert an influence on the quality of P. heterophylla. The purpose of this study is to discriminate P. heterophylla collected from different geographical origins of China. Methods: In this study, the content of polysaccharide in 28 batches of P. heterophylla was determined using phenol-sulfuric acid. HPLC fingerprints were established under optimised HPLC-PDA methods. Subsequently, the similarity analysis (SA) and the quantification of heterophyllin B were analyzed. The metabolites of P. heterophylla were identified and evaluated using UHPLC-Q Exactive HF orbitrap MS system. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), hierarchical cluster analysis (HCA) and orthogonal PLS-DA (OPLS-DA) were performed based on all peak areas. Results: The polysaccharide content in Guizhou and Jiangsu was higher than that of other production areas, which varied significant from different origins. While the content of heterophyllin B in Anhui and Jiangsu was high. The correlation coefficients of HPLC fingerprints for 28 batches samples ranged from 0.877 to 0.990, and the characteristic map can be used to identify and evaluate the quality of P. heterophylla. The samples from Fujian, Guizhou, Jiangsu provinces can be relatively separated using multivariate statistical analysis including PCA, PLS-DA, HCA, OPLS-DA, indicating that their metabolic compositions were significantly different. Ultimately, a total of 15 metabolites which were filtrated by a VIP-value > 1 and a P-value ＜ 0.05 associated with the separation of different origins were identified. Conclusion: HPLC fingerprint was established to evaluate the quality and authenticity of P. heterophylla. The present work showed that the difference of geographic distributions had an influence on the internal chemical compositions. A sensitive and rapid untargeted metabolomics approach by UHPLC-Q Exactive HF orbitrap MS was utilized to evaluate P. heterophylla from different origins in China for the first time. Overall, this study provides insights to metabolomics of P. heterophylla and supplies important reference values for the development of functional foods.

    關(guān)鍵詞:
    HPLC指紋圖譜;代謝產(chǎn)物;太子參;多糖; UHPLC-Q Exactive HF軌道阱質(zhì)譜系統(tǒng)

    Keywords:


    Project Supported:
    This work was supported by the National Key R&D Program of China (No. 2019YFC1710604), the National Key Research and Development Program (No. 2020YFA0907903), the key Project at Central Government Level (No. 2060302), Tianjin Science and Technology Plan Project (No. 19YFZCSY00170), and the National Natural Science Foundation of China (No. 81872956).

    Clc Number:

WeChat

Mobile website