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Archive > Volume 15 Issue 3 > 2023,15(3):463-469. DOI:https://doi.org/10.1016/j.chmed.2022.08.010 Prev Next

麻瘋樹葉化學成分研究

Original articles

Chemical constituents from leaves of Jatropha curcas

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    摘要:
    目的：研究麻瘋樹葉的化學成分，并評價其對脂多糖（LPS）誘導的BV-2小膠質(zhì)細胞過度活化的抑制作用。 方法：采用大孔吸附樹脂、硅膠、ODS柱色譜和半制備高效液相色譜等方法對麻瘋樹葉正丁醇萃取物進行分離，并通過質(zhì)譜、核磁共振、圓二色譜和其他譜學方法對其進行了鑒定。此外，通過檢測過度激活的BV-2細胞中一氧化氮的釋放來評估分離化合物的抗神經(jīng)炎癥作用。 結(jié)果：分離鑒定了17個化合物，分別為：(7R,8S)-crataegifin A-4-O-β-D-glucopyranoside（1）、(8R,8'R)-牛蒡子苷元（2）、牛蒡子苷（3）、(-)丁香脂素（4）、丁香脂素-4'-O-β-D-吡喃葡萄糖（5）、(-)-松脂醇（6）、松脂醇-4'-O-β-D-吡喃葡萄糖（7）、buddlenol D（8）、(2R,3R)-二氫槲皮素（9）、(2S,3S)-表兒茶酸（10）、(2R,3S)-兒茶酸（11）、異牡荊素（12）、柚皮苷（13）、狼毒素（14）、新狼毒素 B（15）、異新狼毒素A（16）和tomentin-5-O-β-D-glucopyranoside (17)?；衔?、4和8顯著抑制LPS激活的BV-2小膠質(zhì)細胞一氧化氮的釋放，IC50值分別為18.34、29.33和26.30 μmol/L。 結(jié)論：化合物1為未見報道的新化合物，化合物2、3、8、14 ~ 17為首次從麻瘋樹屬植物中分離得到。此外，還發(fā)現(xiàn)木脂素類成分對一氧化氮的釋放有明顯的抑制作用，但其成苷后抑制活性降低。

    Abstract:
    Objective: To investigate the chemical constituents from the leaves of Jatropha curcas and evaluate their inhibition on lipopolysaccharide (LPS)-activated BV-2 microglia cells. Methods: The n-BuOH extract of the leaves of J. curcas was isolated by macroporous adsorption resin, silica gel, ODS, column chromatography and semi-preparative HPLC. The structures of the compounds were identified by MS, NMR, ECD, and other spectroscopic methods. In addition, anti-neuroinflammatory effects of isolated compounds were evaluated by measuring the production of nitric oxide (NO) in over-activated BV-2 cells. Results: Seventeen compounds, including (7R,8S)-crataegifin A-4-O-β-D-glucopyranoside (1), (8R,8'R)-arctigenin (2), arctigenin-4'-O-β-D-glucopyranoside (3), (-)-syringaresinol (4), syringaresinol-4'-O-β-D-glucopyranoside (5), (-)-pinoresinol (6), pinoresinol-4'-O-β-D-glucopyranoside (7), buddlenol D (8), (2R,3R)-dihydroquercetin (9), (2S,3S)-epicatechin (10), (2R,3S)-catechin (11), isovitexin (12), naringenin-7-O-β-D-glucopyranoside (13), chamaejasmin (14), neochamaejasmin B (15), isoneochamaejasmin A (16), and tomentin-5-O-β-D-glucopyranoside (17) were isolated and identified. Compounds 2, 4 and 8 significantly inhibited the release of NO in BV-2 microglia activated by LPS, with IC50 values of 18.34, 29.33 and 26.30 μmol/L, respectively. Conclusion: Compound 1 is a novel compound, and compounds 2, 3, 8, 14–17 are isolated from Jatropha genus for the first time. In addition, the lignans significantly inhibited NO release and the inhibitory activity was decreased after glycosylation.

    關(guān)鍵詞:
    抗神經(jīng)炎癥;黃酮;麻瘋樹;木脂素

    Keywords:


    Project Supported:
    This work was financially supported by National Natural Science Foundation of China (No. 81872768, U1903122), Shenyang Young Scientific and Technological Innovators Program (No. RC200408), Doctoral Scientific Research Foundation of Liaoning Province (No. 2020-BS-129).

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