Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro

doi:https://doi.org/10.1016/j.chmed.2023.01.006

Archive > Volume 15 Issue 4 > 2023,15(4):533-541. DOI:https://doi.org/10.1016/j.chmed.2023.01.006 Prev Next

巨傘芋中β-豆甾醇、麥角甾醇和木糖酸的分離、表征及其對(duì)線粒體通透性轉(zhuǎn)換孔的體外調(diào)節(jié)作用

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Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro

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摘要:

目的：藥用植物次生代謝物和多酚類(lèi)化合物已被證實(shí)具有多種生物學(xué)功能，具有廣闊的研究開(kāi)發(fā)前景。本研究考察了從巨傘芋中分離的β-豆甾醇 (含麥角甾醇) 和木糖酸對(duì)肝臟線粒體通透性轉(zhuǎn)換孔(mPTP)的影響。方法：采用真空液相色譜法分離化合物。線粒體腫脹通過(guò)琥珀酸激活條件下吸光度的變化來(lái)評(píng)估。結(jié)果：經(jīng)1H和13C核磁共振鑒定，證實(shí)該分離物中含有β-豆甾醇、麥角甾醇 (1:0.3) 和木糖酸。分離物逆轉(zhuǎn)了脂質(zhì)過(guò)氧化的增加，抑制了鈣和葡萄糖引起的線粒體通透性轉(zhuǎn)換孔的開(kāi)放。mPTP的藥理學(xué)抑制為治療線粒體相關(guān)疾病提供了一個(gè)有前景的治療靶點(diǎn)。結(jié)論：觀察到鈣腺苷三磷酸酶活性的降低以及Caspase-3和Caspase-9的表達(dá)降低，表明它們可能在保護(hù)膜雙分子層的物理化學(xué)性質(zhì)免受自由基誘導(dǎo)的嚴(yán)重細(xì)胞損傷中發(fā)揮作用，并在大量細(xì)胞凋亡發(fā)生的疾病的治療中發(fā)揮作用。

Abstract:

Objective: Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of β-stigmasterol (with ergosterol) and xylopic acid isolated from Anchomanes difformis on liver mitochondrial permeability transition pore (mPTP). Methods: The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions. Results: 1H and 13C NMR spectroscopic elucidation of the isolates affirmed the presence of β-stigmasterol with ergosterol (1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose. Pharmacological inhibition of mPTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders. Conclusion: Reduction in the activity of calcium ATPase and the expression of Caspase-3 and-9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.

關(guān)鍵詞:

巨傘芋;麥角甾醇;線粒體膜;調(diào)制;β-豆甾醇;轉(zhuǎn)換孔;木糖酸

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