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Archive > Volume 15 Issue 4 > 2023,15(4):542-548. DOI:https://doi.org/10.1016/j.chmed.2023.03.007 Prev Next

燈盞乙素通過抑制體內(nèi)外肝細(xì)胞凋亡減輕2型糖尿病肝損傷

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Scutellarin alleviates liver injury in type 2 diabetic mellitus by suppressing hepatocyte apoptosis in vitro and in vivo

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    摘要:
    目的：燈盞乙素是燈盞花的主要活性成分。眾所周知，燈盞乙素具有抗炎、抗氧化的生理功能。本研究檢測(cè)了燈盞乙素對(duì)2型糖尿?。═2DM）大鼠肝細(xì)胞凋亡的影響。 方法：將SD大鼠（6 ~ 8周，160 ~ 180 g）隨機(jī)分為6組：對(duì)照組、模型組、燈盞乙素低劑量[50 mg/(kg·d)]、中劑量[100 mg/(kg·d)]、高劑量[200 mg/(kg·d)]組和羅格列酮對(duì)照[5 mg/(kg·d)]組；每組10只SD大鼠（n = 10）。自動(dòng)生化儀檢測(cè)血清生化因子變化，蘇木精伊紅（HE）染色檢測(cè)肝組織病理變化，組織染色和流量分析儀檢測(cè)肝組織和細(xì)胞凋亡情況，qPCR、western blot和免疫組化檢測(cè)肝組織或細(xì)胞凋亡相關(guān)因子表達(dá)。 結(jié)果：燈盞乙素能降低空腹血糖、總膽固醇、甘油三酯、低密度脂蛋白水平，提高高密度脂蛋白水平。同時(shí)，燈盞乙素能夠降低了大鼠谷丙轉(zhuǎn)氨酶（ALT）和天冬氨酸轉(zhuǎn)氨酶（AST）水平，改善了肝功能。此外，燈盞乙素能夠抑制白細(xì)胞介素-1（IL-1）、白細(xì)胞介素-6（IL-6）和腫瘤壞死因子-α（TNF-α）的分泌，減少肝細(xì)胞凋亡。另外，燈盞乙素還抑制了cleaved Caspase-3、Bax和Cyt-C的表達(dá)，促進(jìn)了Bcl-2的表達(dá)。 結(jié)論：燈盞乙素可通過抑制肝細(xì)胞凋亡信號(hào)通路，從而緩解T2DM。

    Abstract:
    Objective: Scutellarin is a primary active composition come from Erigeron breviscapus. It is well known that scutellarin has anti-inflammatory and antioxidant physiological functions. In this study, we detect the effects of scutellarin on hepatocyte cell apoptosis in type 2 diabetes mellitus (T2DM) rats. Methods: Sprague Dawley (SD) (6–8 weeks, 160–180 g) rats were randomly divided into six groups: control, model, scutellarin low-dose, medium-dose, high-dose treatment, and rosiglitazone positive groups; with 10 SD rats in each group (n = 10). The changes of biochemical factors in serum were detected by automatic biochemical instrument, the pathological changes of liver tissue were detected by hematoxylin and eosin (HE) staining, the apoptosis of liver tissue and cells was detected by tissue staining and flow analyzer, and the expression of apoptosis-related factors were determined by qPCR, Western blot and immunohistochemistry in liver tissues or cells. Results: The results showed that scutellarin decreased the levels of fasting blood glucose, total cholesterol, triglyceride, and low-density lipoprotein and increased the levels of high-density lipoprotein. Meanwhile, scutellarin decreased the levels of alanine transaminase (ALT) and aspartate transaminase (AST) and improved liver function. In addition, scutellarin suppressed the secretion of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and reduced hepatocyte apoptosis. Furthermore, scutellarin inhibited the expression of cleaved Caspase-3, Bax, and cytochrome C (Cyt-C) and promoted the expression of Bcl-2. Conclusion: Scutellarin can inhibit the apoptotic pathway, thereby relieving T2DM.

    關(guān)鍵詞:
    細(xì)胞凋亡;肝細(xì)胞細(xì)胞;肝功能衰竭;燈盞乙素;2型糖尿病

    Keywords:


    Project Supported:
    The study was funded by Science and Technology Base and Talent Project of Guangxi Autonomous Region (No. 2020AC20022). 2022 Guilin Self-funded Science and Technology Project (No. 20220129z). 2020 Research Basic Ability Improvement Project of Young and Middle-aged Teachers in Colleges or Universities in Guangxi (No. 2020KY12013).

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