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Archive > Volume 15 Issue 4 > 2023,15(4):549-555. DOI:https://doi.org/10.1016/j.chmed.2023.02.007 Prev Next

小檗堿的抗氧化和抗關(guān)節(jié)炎潛能:體外和體內(nèi)研究

Original articles

Antioxidant and antiarthritic potential of berberine: In vitro and in vivo studies

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    摘要:
    目的：從具芒小檗 (小檗科) 中提取分離小檗堿。對(duì)分離的小檗堿進(jìn)行了光譜學(xué)表征，并對(duì)其抗氧化和抗關(guān)節(jié)炎活性進(jìn)行了分析。 方法：采用微波輔助提取法從具芒小檗中提取小檗堿，并采用波譜技術(shù)對(duì)其進(jìn)行鑒定。分離的小檗堿在DPPH、一氧化氮和超氧化物清除試驗(yàn)中評(píng)估其抗氧化活性，而在完全弗氏佐劑 (CFA) 誘導(dǎo)的關(guān)節(jié)炎大鼠模型中評(píng)估其抗關(guān)節(jié)炎活性。 結(jié)果：小檗堿在DPPH、一氧化氮和超氧化物清除實(shí)驗(yàn)中顯示出強(qiáng)大的抗氧化活性。在CFA誘導(dǎo)的關(guān)節(jié)炎大鼠模型中，小檗堿的體內(nèi)抗關(guān)節(jié)炎活性顯示出爪子直徑、關(guān)節(jié)炎評(píng)分和體重的顯著減少。此外，小檗堿對(duì)血液學(xué)參數(shù)有濃度依賴性的改善作用。報(bào)告了血清中促炎生物標(biāo)志物，包括IL-6、IL-10和TGF-b，組織病理學(xué)檢查顯示小檗堿可減少血管翳形成、滑膜增生和骨侵蝕。射線檢測(cè)顯示小檗堿治療后軟組織炎癥，骨吸收和侵蝕，關(guān)節(jié)間隙縮小，結(jié)締組織大量擴(kuò)張。 結(jié)論：小檗堿對(duì)血液學(xué)參數(shù)和促炎生物標(biāo)志物的改善作用使其成為治療關(guān)節(jié)炎的理想藥物。

    Abstract:
    Objective: To extract and isolate berberine from Berberis aristata (Berberidaceae). Isolated berberine was characterised using spectroscopy and its antioxidant and antiarthritic activity was analyzed. Methods: The berberine was isolated from B. aristata using microwave-assisted extraction (MAE) and characterised by a spectroscopic technique. The isolated berberine was evaluated for its antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays, while antiarthritic activity was evaluated in the complete freund’s adjuvant (CFA)-induced arthritis rat model. Results: The antioxidant activity of berberine revealed potent antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays. The in vivo antiarthritic activity of berberine in the CFA-induced arthritis rat model showed a significant reduction in paw diameter, arthritic score, and an increase in body weight. Furthermore, a concentration-dependent ameliorating action of berberine on haematological parameters was noticed. Proinflammatory biomarkers, including IL-6, IL-10, and TGF-b in serum were reported, and histopathology examination revealed that berberine decreased pannus formation, synovial hyperplasia, and bone erosion. Radiographic investigation showed soft tissue inflammation, bone resorption and erosion, joint gap reduction, and substantial connective tissue expansion after treatment with berberine. Conclusion: The ameliorating action on haematological parameters and proinflammatory biomarkers of berberine makes them a suitable remedy for the treatment of arthritis.

    關(guān)鍵詞:
    抗氧化劑;抗關(guān)節(jié)炎活性;小檗堿;具芒小檗;微波輔助

    Keywords:


    Project Supported:
    Thanks to Dr. P.K. Khare from Department of Botany, Dr. H.S. Gour, Central University, Sagar (M.P.) for identification and confirmation of plant specimens.

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