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Archive > Volume 15 Issue 4 > 2023,15(4):564-573. DOI:https://doi.org/10.1016/j.chmed.2023.02.003 Prev Next

掛金燈對腸道細菌和真菌微生態(tài)系統的調節(jié)作用

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Modulative effect of Physalis alkekengi on both gut bacterial and fungal micro-ecosystem

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    摘要:
    目的：腸道微生物組是一個復雜的微生態(tài)系統，與人體健康狀態(tài)和藥物的療效密切相關。掛金燈（Physalis alkekengi L., PAL）是一種可食用、歷史悠久的中藥。研究表明，PAL具有多種藥理活性，并且腸道細菌在疾病的的治療中也發(fā)揮著重要作用。然而，PAL對腸道細菌和腸道真菌的具體調節(jié)作用在很大程度上仍然未知。因此，我們在正常小鼠中設計了一個初步的實驗，以揭示PAL對腸道細菌和真菌的調節(jié)作用，并探討它們之間的相互作用。 方法：將PAL水提物灌胃給予正常C57BL/6小鼠4周。采用全長16S rRNA和ITS1/2基因測序技術，分別檢測PAL處理后的腸道細菌和腸道真菌群落。 結果：研究結果顯示，口服PAL顯著富集抗炎菌，如Duncaniella spp.和Kineothrix alysoides，而降低了促炎菌，如Mucispirillum schaedleri的相對豐度。同時，PAL增加了真菌Aspergillus ochraceus，Cladosporium sp.和Alternaria sp., 降低了Penicillium janthinellum。相關性網絡分析顯示，兩個共存的微生物組（組1和組2）彼此呈負相關。組1主要為PAL處理小鼠中富集的細菌和真菌，組2主要為正常小鼠中富集的細菌和真菌。組1中，真菌Antrodia monomitica，Aspergillus clavatus，Mortierella kuhlmanii 和Sarcinomyces sp. MA 4787 與細菌Bifidobacterium globosum，Romboutsia ilealis等正相關。組2中，真菌Chaetomium subspirilliferum，Septoria orchidearum和Cephaliophora tropica與細菌 Lactobacillus spp. 正相關。 結論：本研究初步揭示了PAL對腸道細菌和腸道真菌的調節(jié)作用，這可能有助于闡明PAL的藥理機制。

    Abstract:
    Objective: Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi (PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic actions. However, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largelym unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between them. Methods: Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, respectively. Results: Oral administration of PAL notably enriched anti-inflammatory bacterial species such as Duncaniella spp. and Kineothrix alysoides, whereas decreased pro-inflammatory species such as Mucispirillum schaedleri. Simultaneously, PAL increased the abundance of gut fungi Aspergillus ochraceus, Cladosporium sp. and Alternaria sp., and decreased Penicillium janthinellum. Correlation network analysis identified two co-existing microbial groups (groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergillus clavatus, Mortierella kuhlmanii and Sarcinomyces sp. MA 4787 were positively correlated with Bifidobacterium globosum, Romboutsia ilealis and so on. In group 2, Chaetomium subspirilliferum, Septoria orchidearum and Cephaliophora tropica were positively related to Lactobacillus spp. Conclusion: Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechanism.

    關鍵詞:
    腸道細菌;腸道真菌;掛金燈;中藥;微生態(tài)系統

    Keywords:


    Project Supported:
    This work was supported financially by the National Natural Science Foundation of China (No. 81973217).

    Clc Number:

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