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Archive > Volume 15 Issue 4 > 2023,15(4):607-613. DOI:https://doi.org/10.1016/j.chmed.2023.05.005 Prev Next

采用qNMR、FTIR和GC-MS對沙棘果油進(jìn)行比較定量分析

Original articles

Comparative quantitative analysis of fruit oil from Hippophae rhamnoides (seabuckthorn) by qNMR, FTIR and GC–MS

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    摘要:
    目的：建立沙棘 (SBT) 果油中甘油三酯的定量分析方法，并采用氣相色譜-質(zhì)譜聯(lián)用技術(shù)和紅外光譜技術(shù)對市售沙棘果油進(jìn)行分析。 方法：采用己烷萃取法提取SBT果油 (IPHRFH)，采用真空液相色譜法分離甘油三酯 (TAG)。從電子商務(wù)供應(yīng)商 (亞馬遜) 購買的6種不同品牌的SBT油，用qNMR對內(nèi)部SBT油進(jìn)行了分析，用NMR對甘油三脂的脂肪?；M成進(jìn)行了測定，也用GC-MS和FTIR光譜對內(nèi)部油進(jìn)行了分析。 結(jié)果：qNMR結(jié)果表明，該油含有80.3%的甘油三酯 (TAG)。經(jīng)qNMR測定，SBT甘油三酯由6.6%亞麻酸酯，65.4%棕櫚油酸酯/油酸酯和28%含棕櫚酸酯的總飽和脂肪?；溄M成。GC-MS分析顯示，TAG中主要的酰基官能團(tuán)為棕櫚油酸36.5%、油酸12.9%、棕櫚酸21.2%和亞油酸18%。在分析的六個(gè)商業(yè)樣品中，只有一個(gè)供應(yīng)商(SW)的樣品是果油；所有其他的都是種子油或果油和籽油的混合物。除了SW外，其他樣品的標(biāo)簽都沒有標(biāo)明是果油還是籽油。 結(jié)論：結(jié)果表明，SBT油應(yīng)采用GC-MS、FTIR和qNMR相結(jié)合的方法分析其實(shí)際游離脂肪酸或甘油三酯的含量，它們的化學(xué)性質(zhì)不同，會(huì)影響油的質(zhì)量。GC-MS顯示水解后ω游離脂肪酸含量，qNMR和FTIR顯示水解后甘油三酯的含量。在SBT果油甘油三酯中發(fā)現(xiàn)的主要?；倌軋F(tuán)是棕櫚油酸酯/棕櫚酸酯/油酸酯，而亞油酸酯和亞麻酸酯只占一小部分。此外，對商業(yè)樣品的分析顯示標(biāo)簽標(biāo)注與實(shí)際含量之間存在差異。

    Abstract:
    Objective: To develop a qNMR method for quantitative analysis of triacylglycerols in fruit oil of Hippophae rhamnoides (seabuckthorn, SBT) and analyze commercial samples of SBT oils using GC–MS and FTIR. Methods: SBT fruit oil (IPHRFH) was extracted with hexane and the triglyceride (TAG) was isolated by vacuum liquid chromatography. Six different branded SBT oils purchased from e-commerce suppliers (Amazon) and in-house prepared SBT oil was analyzed by qNMR and fatty acyl composition of TAGs determined by using NMR. In-house oil was also analysed by GC–MS and FTIR spectroscopy. Results: The qNMR results showed that the oil contained 80.3% of triacylglycerol (TAG). The SBT oil TAGs comprised of linolenate 6.6%, palmitoleate/oleate 65.4%, and total saturated fatty acyl chain including palmitate 28% as determined by qNMR. GC–MS analysis revealed that the major acyl functionalities present in the TAG were palmitoleic acid 36.5%, oleic acid 12.9%, palmitic acid 21.2%, and linoleic acid 18%. Of the six commercial samples analyzed, samples from only one supplier (SW) were fruit oil; All others were the seed oils or mix of fruit oil and seed oil. The labels for samples except for the SW did not indicate whether it was fruit oil or seed oil. Conclusion: The results suggest that SBT oil should be analyzed by combination of GC–MS, FTIR and qNMR for factual content of free fatty acid or TAGs, which are chemically different in nature and affect the quality of oil. GC–MS showed the content of omega free fatty acids after hydrolysis, while qNMR and FTIR showed the content of TAGs. The major acyl functionalities found in SBT fruit oil TAGs are palmitoleate/palmitate/oleate, while linoleate and linonelate make up a minor fraction. Furthermore, analysis of commercial samples showed discrepancies between label claims and actual content.

    關(guān)鍵詞:
    沙棘;ω脂肪酸;qNMR;沙棘;甘油三酯

    Keywords:


    Project Supported:
    Authors are thankful to Department of Biotechnology, Government of India for a research grant (DBT-New Delhi, No. BT/ PR9116/NDB/39/390/2013). Authors are thankful to Director NIPER S.A.S. Nagar and Director CSIR-IHBT Palampur for providing research facilities.

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