目的 建立山楂藥材HPLC指紋圖譜，為有效控制山楂藥材的質(zhì)量奠定基礎(chǔ)。方法 采用HPLC法，色譜柱為Agilent TC-C₁₈柱(250 mm×4.6 mm,5 μm)，乙腈-0.01%甲酸水梯度洗脫，檢測(cè)波長為280 nm，體積流量為1.0 mL/min，分析時(shí)間為120 min，分析了10批山楂藥材的HPLC指紋圖譜。結(jié)果 在選定的色譜條件下，通過相似度分析確定23個(gè)色譜峰構(gòu)成山楂藥材指紋圖譜的特征峰。結(jié)論 采用HPLC方法建立的指紋圖譜具有精密、穩(wěn)定、重現(xiàn)性好的特點(diǎn)，可用于山楂藥材的質(zhì)量控制。

Objective To study the fingerprints of Fructus Crataegus by HPLC and establish a sensitive, specific method for quality control. Methods The gradient elution mode was applied in chromatographic separation: Agilent TC-C₁₈ column (250 mm×4.6 mm, 5μm) was used with the mobile phase of acetonitrile-water (0.01% formic acid), UV detection wavelength of 280 nm, flow rate of 1.0 mL/min, analysis time of 120 min. Ten batches of Fructus Crataegus were studied. Results 23 Peaks were identified as the characteristic fingerprints of Fructus Crataegus. Conclusion The established method shows satisfactory accuracy, stability, and reproducibility, and can by used as a quality control method for Fructus Crataegus.

“十一五”國家科技支撐計(jì)劃（2006BAI08B03-04）