目的 探討竹節(jié)香附素A對人肝癌細胞 HepG₂增殖的影響。方法 將不同質量濃度的竹節(jié)香附素A與 HepG₂細胞共同培養(yǎng)，采用 MT T比色法及細胞集落形成法檢測竹節(jié)香附素A對HepG₂細胞生長、增殖的影響。結果 竹節(jié)香附素A對 HepG₂ 細胞生長的 IC_５０ 為 894 μg/mL。與未處理組相比，5、10、20、40 μg/mL 的竹節(jié)香附素A分別與 HepG₂ 細胞共同培養(yǎng) 72 h，在培養(yǎng) 24 h 時的細胞增殖抑制率分別為：(24.81±0.55)%、(39.17±1.30)%、(62.45±7.56)%、(79.93±5.48)%，在培養(yǎng) 48 h 時的抑制率為 (35.67±0.81)%、(49.73±9.18)%、(71.62±1.03)%、(87.06±1.84)%，在培養(yǎng) 72 h 時的抑制率為 (42.52±1.31)%、(59.75±7.47)%、(78.85±3.15)%、(91.36±0.84)%；順鉑對照組在細胞培養(yǎng) 24、48、72 h 的抑制率分別為 (2.60±0.53)%、(60.55±5.66)%、(82.61±8.95)%。竹節(jié)香附素A各劑量的作用與未處理組相比，在各時間點均有顯著差異 (P＜0.001)；與順鉑相比，竹節(jié)香附素A起效更快，24 h 檢測具有顯著差異 (P＜0.001)；5、10、20 μg/mL 劑量在 24 h 以外的時間點與順鉑相比無差異，40 μg/mL 劑量在各時間點對 HepG₂ 細胞的抑制作用仍較順鉑的作用強 (P＜0.01)。集落形成實驗顯示，對照組的集落形成率為 45.47%；而竹節(jié)香附素A 1.25、2.5、5 μg/mL 3個劑量組僅分別為 15.73%、7.2%、3.33%，即集落形成抑制率分別為 (66.75±1.66)%、(84.25±3.11)%、(92.55±2.33)%，與對照組相比有顯著差異 (P＜0.001)。結論 竹節(jié)香附素A對 HepG₂ 細胞增殖有明顯抑制作用，在一定范圍內隨著試藥質量濃度的升高和作用時間的延長而增強。

Objective To investigate the effect of raddeanin A on the proliferation of human hepatic cancer cells HepG₂. Methods Human hepatic cancer cells HepG₂ were cocultured with different concentrations of raddeanin A, the effect of raddeanin A on the growth of HepG₂ cells was examined using MTT assay and colonogenic assay. Results Raddeanin A against the HepG2 cells with IC_50 of 8.94 μg/mL. HepG₂ cells were co-cultured with 5, 10, 20, and 40 μg/mL raddeanin A for 72 h, against the cells proliferation with inhibitory rate of (24.81±0.55)%, (39.17±1.30)%, (62.45±7.56)%, and (79.93±5.48)% at 24 h; (35.67±0.81)%, (49.73±9.18)%, (71.62±1.03)%, and (87.06±1.84)% at 48 h; (42.52±1.31)%, (59.75±7.47)%, (78.85±3.15)%, and (91.36±0.84)% at 72 h, respectively, compared with control group. Cisplatin showed the inhibitory rate of (2.60±0.53)%, (60.55±5.66)%, and (82.61±8.95)%, respectively. Each dose group in comparison with the vehiculum group, were significant differences in various time point (P＜0.001). In comparison with cisplatin group, raddeanin A inhibited more rapidly the proliferation of HepG₂ cells at 24 h (P＜0.001). Other concentrations of the compound except at 4.0 μg/mL, have no difference compared with cisplatin, at different times point. Effect of raddeanin A 40 μg/mL against HepG₂ cells proliferation was still stronger than cisplatin (P＜0.01) at different times point. Colonogenic assay demonstrated that the colonyforming rate were 15.73%, 7.2%, and 3.33%, respectively, vizcolonyforming inhibitory rate were (66.75±1.66)%, (84.25±3.11)%, and (92.55±2.33)%, respectively, compared with the control group were significantly different (P＜0001).Conclusion Raddeanin A showed significanly inhibitory on proliferation of HepG2 cells in vitro, and at certain range the inhibitory effects was depended on the medicine concentration and treating times.