目的 從川西獐牙菜藥材中制備高純度獐牙菜苦苷。方法 川西獐牙菜經(jīng)醇提水沉后，上清液經(jīng)HPD-300大孔吸附樹脂吸附洗脫后得獐牙菜苦苷粗提物，再用閃式柱色譜對(duì)其進(jìn)行分離。結(jié)果 制備得到的獐牙菜苦苷，純度在90%以上，工藝總收率為79.94%。結(jié)論 本實(shí)驗(yàn)建立的制備高純度的獐牙菜苦苷的方法，可操作性強(qiáng)，重現(xiàn)性較好，工藝收率和產(chǎn)品純度均較為理想。

Objective To prepare swertiamarin with high purity from Swertia mussotii Franch. with macroporous resin and flash chromatography. Methods Herba S. Mussotii was extracted with ethanol solvent. The extract solvent was adsorpted and separated with HPD-300 to obtain crude extract of swertiamarin， then the crude extract is purified with flash chromatography.Results The purity of swertiamarin was more then 90%; recovery rate was 79.94%. Conclusion The preparation method established in this experiment can be operated easily with good reproducibility and high recovery rate. The purity of the swertiamarin can be up to 90%.

天津市自然基金項(xiàng)目（08JCZDJC24700和10JC2DJC21400）