目的 研究仙鶴草醋酸乙酯有效部位（總鞣質(zhì)質(zhì)量分?jǐn)?shù)為19.91%）對(duì)人肝癌HepG2細(xì)胞增殖的抑制作用及其機(jī)制。方法 以不同質(zhì)量濃度的仙鶴草醋酸乙酯有效部位作用于體外培養(yǎng)的HepG2細(xì)胞，MTT法檢測(cè)細(xì)胞生長(zhǎng)抑制率；流式細(xì)胞儀檢測(cè)細(xì)胞凋亡；Fluo-3/AM熒光探針觀(guān)察腫瘤細(xì)胞內(nèi)鈣離子濃度的變化；流式細(xì)胞儀檢測(cè)腫瘤細(xì)胞內(nèi)活性氧（ROS）的變化。結(jié)果 仙鶴草醋酸乙酯有效部位可顯著抑制HepG2細(xì)胞的生長(zhǎng)，誘導(dǎo)細(xì)胞凋亡，其IC50為127.85 μg/mL。經(jīng)該有效部位作用48 h后，HepG2細(xì)胞數(shù)量明顯減少；在Fluo-3/AM熒光探針的作用下有較強(qiáng)的綠色熒光；同時(shí)檢測(cè)出細(xì)胞內(nèi)ROS有較明顯的增加。結(jié)論 仙鶴草醋酸乙酯有效部位能抑制HepG2細(xì)胞的生長(zhǎng)，誘導(dǎo)細(xì)胞發(fā)生凋亡。腫瘤細(xì)胞內(nèi)鈣離子的釋放和細(xì)胞內(nèi)ROS的增加可能是其作用機(jī)制。

Objective To study the antiproliferation effects of extract from Agrimonia pilosa（tannin 19.91%）on hepatocellular carcinoma HepG2 cells and their mechanisms. Methods HepG2 cells in culture medium were treated with different concentrations of extract of A. pilosa. The inhibitory rate of the cells was measured by MTT assay. Cell apoptotic rate was detected by flow cytometry (FCM). Fluo-3/AM fluorescent probe was used to observe the Ca2+ variation in the tumour cells. The variation of intracellular ROS was detected by FCM. Results The extract of A. pilosa could inhibit the growth of HepG2 cells and cause apoptosis significantly. The IC50 was 127.85 μg/mL. The number of cells was decreased especially after the cells were treated 48 h by the extract of A. pilosa,and the green fluorescence was seen clearly by Fluo-3/AM fluorescent probe. The intracellular ROS was increased obviously. Conclusion The extract of A. pilosa could inhibit the growth of HepG2 cells and cause apoptosis. The releasing of the intracellular Ca2+ variation and the increasing of the intracellular ROS may be its anti-hepatocellular carcinoma mechanisms.

黑龍江省留學(xué)回國(guó)人員重點(diǎn)科技項(xiàng)目（黑人發(fā)[2009]23號(hào)）