甘草因療效顯著、藥理作用明確，一直以來(lái)都是研究的熱點(diǎn)。近年來(lái)有20個(gè)黃酮類(lèi)化合物和6個(gè)三萜類(lèi)化合物被陸續(xù)分離出來(lái)，其中包括16個(gè)新的黃酮類(lèi)化合物和3個(gè)新的三萜類(lèi)化合物。細(xì)胞培養(yǎng)是獲得甘草有效成分的有效途徑之一，目前應(yīng)用細(xì)胞培養(yǎng)技術(shù)主要生產(chǎn)甘草黃酮類(lèi)成分。綜述了近年來(lái)從甘草中提取的黃酮類(lèi)、三萜類(lèi)化合物，細(xì)胞培養(yǎng)方法對(duì)甘草黃酮類(lèi)成分的影響，以及毛細(xì)管電色譜法、HPLC法、免疫測(cè)定法等檢測(cè)甘草化學(xué)成分方法的研究進(jìn)展。

Glycyrrhizae Radix et Rhizoma has been the research focus because of its conspicuous healing efficacy and pharmacological activities. Recently, 20 flavonoids and 6 triterpenes compounds, including 16 new flavonoids and 3 new triterpenes have been isolated from this plant. It was also found that cell culture provided an effective way to acquire effective components of Glycyrrhizae Radix et Rhizoma. The cell culture technique is mainly used for product Glycyrrhizae Radix et Rhizoma flavonoids. In this paper, the recent progresses was reported on the chemical components of the plant, the cell culture method for the bioactive flavonoids ingredients and determination of its effective constituents using capillary electrochromatography, HPLC, and immunoassays detection as well.

“十一?五”國(guó)家科技重大專(zhuān)項(xiàng)資助項(xiàng)目（2009ZX09501-011）；新疆維吾爾自治區(qū)科學(xué)技術(shù)廳資助項(xiàng)目（200915122）