綜述了近年來淫羊藿苷的提取分離方法和抗腫瘤活性及其機(jī)制。目前報(bào)道的提取方法有醇提法、超聲法、微波法、高壓法，分離方法有柱色譜法（聚酰胺法和大孔吸附樹脂法）、聚酰胺色譜聯(lián)用醋酸乙酯萃取法等。淫羊藿苷及其衍生物對(duì)肺癌、胃癌、肝癌、乳腺癌、白血病細(xì)胞均有一定的抑制活性，主要作用機(jī)制為淫羊藿苷通過影響腫瘤細(xì)胞周期，下調(diào)與腫瘤細(xì)胞有關(guān)的信號(hào)通路及細(xì)胞因子的表達(dá)，抑制腫瘤細(xì)胞的增殖與轉(zhuǎn)移，最終使腫瘤細(xì)胞凋亡。

The methods for the extraction and separation of icariin, and the mechanism of anti-tumor activity of icariin are reviewed in this paper. There are various extraction and separation methods have been reported, such as ethanol extraction, ultrasonic, microwave, and high pressure; column chromatography (polyamide and macroporous resin adsorption methods), polyamide chromatography combined with ethyl acetate extraction and so on. Icariin and its derivatives show the potential inhibition on several tumor cells, such as lung cancer, gastric cancer, liver cancer, breast cancer, and leukemia cells. Previous study showed that icariin could induce the cell apoptosis through arresting the cell cycle, inhibiting the tumor cell signaling pathways, down-regulating cell factor,expression, and suppressing the tumor cell proliferation and metastasis.