研究板藍(lán)根乙醇提取物的醋酸乙酯和正丁醇萃取層中的化學(xué)成分。方法 采用反復(fù)硅膠柱色譜、凝膠柱色譜法進(jìn)行分離純化，根據(jù)化合物的理化性質(zhì)和波譜數(shù)據(jù)鑒定化合物的結(jié)構(gòu)。結(jié)果 分離鑒定了12個(gè)化合物，分別為喜果苷（1）、quinovic acid-3β-O-β-D-glucopyranosyl-(28→1)-β-D-glucopyranosyl ester（2）、quinovic acid-3β-O-β-D-glucopyranosyl（3）、棕櫚酸甲酯（4）、水楊酸（5）、遠(yuǎn)志醇（6）、腺苷（7）、靛玉紅（8）、靛藍(lán)（9）、大黃素（10）、β-谷甾醇（11）和β-胡蘿卜苷（12）。結(jié)論 化合物1、2、3為首次從十字花科植物中分離得到，化合物4為首次從菘藍(lán)屬植物中分離得到。

To study the chemical constituents in acetic ether and n-butyl alcohol fractions from the roots of Isatis indigotica alcohol extract. Methods The compounds were isolated by a combination of silica gel column and ODS column chromatography. Physicochemical characteristics and spectroscopic evidences were employed for the structural identification. Results Twelve compounds were isolated and identified as vincoside-lactam (1), quinovic acid-3β-O-β-D-glucopyranosyl-(28→1)-β-D- glucopyranosyl ester (2), quinovic acid-3β-O-β-D-glucopyranoside (3), methyl palmitate (4), salicylic acid (5), polygalitol (6), adenosine (7), indirubin (8), indigotin (9), emodin (10), β-sitosterol (11), and β-daucosterol (12). Conclusion Compounds 1, 2, and 3 are identified for the first time from the plants in family Cruciferae, compound 4 is identified for the first time in the plants of Isatis L.