[關(guān)鍵詞]
[摘要]
探討肉桂醛抗煙曲霉的作用機(jī)制。方法 采用固體插片培養(yǎng)法培養(yǎng)煙曲霉,定時(shí)取樣品于光學(xué)顯微鏡下動(dòng)態(tài)觀察煙曲霉生長(zhǎng)情況,以RT-PCR檢測(cè)在肉桂醛作用下煙曲霉FKS基因表達(dá)的變化。結(jié)果 以肉桂醛0.025 μg/mL作用后,煙曲霉生長(zhǎng)顯著遲緩,分生孢子頭稀疏;煙曲霉FKS基因的表達(dá)受到抑制,但隨培養(yǎng)時(shí)間的延長(zhǎng),該抑制作用減弱。結(jié)論 肉桂醛通過(guò)抑制細(xì)胞壁主要多糖合成的關(guān)鍵基因FKS表達(dá),使煙曲霉細(xì)胞壁失去正常生理結(jié)構(gòu),細(xì)胞生長(zhǎng)、繁殖被抑制,從而達(dá)到抗煙曲霉的目的。
[Key word]
[Abstract]
To discover the anti-aspergilli mechanism of cinnamaldehyde on Aspergillus fumigatus. Method Using Czapak's solid petri dish containing glass coverslip, its growth and propagattion were observed timely by light microscope, RT-PCR was performed to detect the effect of cinnamaldehyde on gene expressions of FKS. Results A. fumigatus treated by cinnamaldehyde at concentration of 0.025 μg/mL showed obviously slow growth rate and markly reduced conidial head produced; cinnamaldehyde could inhibit the gene expressions of FKS, and this effect became weaker over incubation time. Conclusion Cinnamaldehyde could inhibit the gene expression of key gene FKS and damage the cell wall structure, which and would be one of its anti-aspergilli mechanism.
[中圖分類號(hào)]
[基金項(xiàng)目]