建立HPLC法測(cè)定腎康顆粒中延胡索乙素的方法。方法 采用Kromasil C18柱（250 mm×4.6 mm，5 μm）；以甲醇–乙腈–1%醋酸銨緩沖液（pH 5.0±0.02）（12∶28∶60）為流動(dòng)相，體積流量1.0 mL/min，檢測(cè)波長(zhǎng)283 nm。結(jié)果 延胡索乙素在0.077 76～1.555 20 μg線(xiàn)性良好（r=0.999 9），樣品平均加樣回收率和RSD值分別為99.6%、1.91%。結(jié)論 此方法簡(jiǎn)便、準(zhǔn)確、重現(xiàn)性良好，可用于腎康顆粒中延胡索乙素的測(cè)定。

Objective To establish an HPLC method for determination of tetrahydropalmatine in Shenkang Granula. Methods Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methol-acetonitrile-1% ammonium acetate (pH 5.0±0.02) (12∶28∶60). The flow rate was 1.0 mL/min and the detection wavelength was at 283 nm. Results The linear range of tetrahydropalmatine was 0.077 76—1.555 20 μg (r=0.999 9). The average recovery was 99.6% and RSD was 1.91%. Conclusion This method is simple, precise, and with good repeatability, and can be used to determine tetrahydropalmatine in Shenkang Granula.