目的 采用高速逆流色譜法分離制備多花蒿中阿格拉賓。方法 多花蒿乙醇提取物經(jīng)硅膠柱色譜分離，所得組份Fr. 1用高速逆流色譜進(jìn)一步分離，采用正己烷–醋酸乙酯–甲醇–水（1.2∶0.8∶1.5∶0.5）為兩相溶劑系統(tǒng)，上相為固定相，下相為流動(dòng)相，主機(jī)轉(zhuǎn)速850 r/min，體積流量2.0 mL/min，檢測(cè)波長(zhǎng)為254 nm。采用波譜法對(duì)所得化合物進(jìn)行結(jié)構(gòu)鑒定，HPLC法測(cè)定產(chǎn)品的純度。結(jié)果 從500 mg組分Fr. 1中得到260 mg質(zhì)量分?jǐn)?shù)為99.5%的阿格拉賓。結(jié)論 該方法操作簡(jiǎn)單，可用于阿格拉賓化學(xué)對(duì)照品的分離制備。

Objective To establish a method for the isolation and preparation of arglabin from Artemisia myriantha by high speed counter-current chromatography (HSCCC). Methods The ethanol extract of A. myriantha was separated with silica gel column chromatography, and the obtained Fr. 1 was further separated by HSCCC with a two-phase solvent system composed of n-hexane-ethylacetate-methanol-water (1.2:0.8:1.5:0.5). The lower phase was used as mobile phase, and the upper phase was used as the stationary phase. Main engine speed was 850 r/min, and the detection wavelength was 254 nm at a flow rate of 2.0 mL/min. The obtained fraction was identified by spectral analysis and analyzed by HPLC. Results A total of 260 mg arglabin with the purity of 99.5% was successfully obtained from 500 mg Fr. 1. Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of arglabin reference substance.

科技部“十一五”重大新藥創(chuàng)制項(xiàng)目（2010ZX09401-402）