目的 優(yōu)化北蒼術(shù)多糖的超聲提取工藝。方法 利用超聲波提取北蒼術(shù)根莖中的多糖，以提取物得率和多糖提取率為指標(biāo)，在單因素試驗(yàn)的基礎(chǔ)上，采用4個(gè)因素（超聲時(shí)間、料液比、超聲溫度、乙醇用量）3個(gè)水平的L9(34)正交試驗(yàn)優(yōu)選超聲提取工藝條件，并采用苯酚–濃硫酸法測(cè)定多糖。結(jié)果 北蒼術(shù)多糖的最佳提取工藝條件為：料液比1∶20、提取溫度60 ℃、超聲提取40 min、濃縮后加入4倍量乙醇進(jìn)行醇沉。在該實(shí)驗(yàn)條件下，蒼術(shù)水提物得率為23.16%，多糖提取率為11.23%。結(jié)論 超聲法提取蒼術(shù)中多糖簡(jiǎn)便快捷，多糖提取率高，為北蒼術(shù)多糖的開發(fā)利用提供了參考。

Objective To optimize the extraction technology of polysaccharide from Atractylodes chinensis using ultrasonic extraction method by orthogonal test. Methods Polysaccharide was extracted from A. chinensis by ultrasonic-assisted extraction method. The yield of crude polysaccharide of A. chinensis and extraction rate of polysaccharide were taken as indices. Based on single-factor experiment, the ultrasonic-assisted extraction technology of polysaccharide from A. chinensis was optimized by L9(34) orthogonal test. The phenol-sulphuric acid method was applied for the determination of polysaccharide. Results The optimal extraction conditions of polysaccharide from A. chinensis were as follows: the solid-liquid ratio was 1∶20; the extraction temperature was 60 ℃; the extraction time was 40 min and alcohol volume was 4 times of the medicinal materials. Under the conditions, the yield crude polysaccharide and the purity of polysaccharide were 23.16% and 11.23%, respectively. Conclusion Ultrasonic extraction has the advantages of short time, simple operation, and higher extract rate, which could provide the reference for development and utilization of A. chinensis.

天津市科技攻關(guān)項(xiàng)目（06YFGPSH02900）