目的 建立脹果甘草藥渣中黃酮類成分的超高效液相色譜–高分辨飛行時(shí)間質(zhì)譜（UPLC-TOF-MS）定性分析方法。方法 Agilent SB-C18柱（100 mm×4.6 mm，1.8 μm）；流動(dòng)相乙腈–0.1%甲酸水溶液，梯度洗脫；體積流量0.4 mL/min；柱溫25 ℃；檢測波長254 nm。ESI離子源，飛行時(shí)間質(zhì)譜檢測器。對(duì)比自制對(duì)照品進(jìn)行鑒別。結(jié)果 共鑒定出8個(gè)黃酮類成分，分別為2',4,4'-三羥基查耳酮、甘草查耳酮D、甘草查耳酮甲、4'-羥基-2'',2''-二甲基吡喃[5'',6'',6,7]黃酮、甘草黃酮C、光甘草酮、甘草黃酮B和kanzonol E。結(jié)論 建立了一種簡單、可靠的UPLC-TOF-MS方法對(duì)脹果甘草藥渣中黃酮類成分進(jìn)行了鑒定，對(duì)脹果甘草藥渣綜合利用有一定參考價(jià)值。

Objective To establish the identification method for flavonoids in gruffs of flavonoids in G. inflate by UPLC-TOF-MS. Methods The method of identification was performed on Agilent SB-C18 column (100 mm×4.6 mm, 1.8 μm), the mobile phase consisted of acetonitrile and 0.1% formic acid aqueous solution with gradient elution program. The flow rate was 0.40 mL/min, the column temperature was 25 ℃, and the wavelength was set at 254 nm. The detector was TOF Mass Spectrometer with an ESI ion source. The data were compared with those of the reference substances. Results Eight flavonoids were identified from the gruffs in G. inflate and were identified as 2',4,4'-trihydroxychalcone, licochalcone D, licochalcone A, 4'-hydroxy-2'',2''-dimethylpyrano [5'',6'',6,7] flavone, licoflavone C, glabrone, licoflavone B, and kanzonol E. Conclusion The established method for the determination of flavonoids in the gruffs of G. inflate using UPLC-TOF-MS is simple, reliable, and could provide the reference on comprehensive utilization.

國家自然科學(xué)基金資助項(xiàng)目（81001371）；新疆維吾爾自治區(qū)高技術(shù)研究發(fā)展項(xiàng)目（200915122）；烏魯木齊市科技計(jì)劃項(xiàng)目（P09111003，Y101120002）