目的 研究芙蓉菊多糖的體外抗氧化活性。方法 采用1,1-二苯基苦基苯肼（DPPH）自由基、還原力、超氧陰離子、羥自由基4種體外抗氧化模型研究芙蓉菊多糖的抗氧化活性，用維生素C作對(duì)照。結(jié)果 芙蓉菊粗多糖對(duì)DPPH自由基、還原力、超氧陰離子、羥自由基均有明顯的清除能力，其中DPPH、超氧陰離子、羥自由基的EC50值分別為0.273、0.669、0.594 mg/mL，對(duì)羥自由基清除能力強(qiáng)于維生素C。芙蓉菊多糖對(duì)自由基清除率與其質(zhì)量濃度存在著明顯的量效關(guān)系。結(jié)論 芙蓉菊多糖具有良好的體外抗氧化活性，作為天然抗氧化劑和自由基清除劑有進(jìn)一步研究的價(jià)值。

Objective To study the in vitro antioxidative activity of Crossostephium chinense polysaccharide (CCPS). Methods Four models of in vitro anti-oxidative activity were used to study, including DPPH, reducing power, superoxide anion radicals, and hydroxyl free radicals, compared with vitamin C. Results CCPS had the evident free radical scavenging capacities on DPPH, reducing power, superoxide anion radicals, and hydroxyl free radicals, and their EC50 values on DPPH, superoxide anion radicals, and hydroxyl free radicals were 0.296, 0.669, and 0.594 mg/mL, respectively. Hydroxyl scavenging capacity of CCPS was higher than that of vitamin C. There was a good linear relationship between the concentration and free radical scavenging capacity of CCPS. Conclusion CCPS has a good in vitro anti-oxidative activity and it deserves to be further studied as a natural anti-oxidant and free radical scavenger.

國(guó)家星火計(jì)劃項(xiàng)目（2012GA700194）