目的 建立女金糖漿中橙皮苷、黃芩苷和丹皮酚的HPLC分析方法。方法 采用資生堂Spolar-C18色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相為[甲醇–乙腈–0.1%磷酸（12∶13∶75）]–乙腈，進(jìn)行梯度洗脫；柱溫40 ℃；體積流量1.0 mL/min；檢測(cè)波長(zhǎng)283 nm；進(jìn)樣量10 μL。結(jié)果 橙皮苷、黃芩苷和丹皮酚分別在0.073 95～2.218 49、0.508 20～10.164 00、0.158 71～2.380 60 μg線性關(guān)系良好，回收率分別為97.0%、98.9%、99.0%，RSD值分別為0.33%、0.41%、0.14%（n=6）。結(jié)論 該方法方便、快速、靈敏度高，可以為女金糖漿的質(zhì)量控制提供依據(jù)。

Objective To establish an HPLC method for the determination of hesperidin, baicalin, and paeonol in Nüjin Syrup. Methods The samples were separated on Shiseido Spolar-C18 column (250 mm×4.6 mm, 5 μm) with [methanol-acetonitrile-0.1% phosphoric acid (12∶13∶75)]-acetonitrile as mobile phase by gradient elution. The detection wavelength was 283 nm and column temperature was 40 ℃ with injection volume of 10 μL at a flow rate of 1.0 mL/min. Results The calibration curves were linear over the range of 0.073 95—2.218 49 μg, 0.508 20—10.164 00, and 0.158 71—2.380 60 μg (r=0.999 1) for hesperidin, baicalin and paeonol. The average recovery rates were 97.0%, 98.9%, and 99.0% with RSD at 0.33%, 0.41%, and 0.14%, respectively (n=6). Conclusion This method is simple, rapid, and sensitive, and could provide the basis for the quality control of Nüjin Syrup.