目的 建立HPLC法同時測定心腦絡(luò)通顆粒中野黃芩苷和4,5-二-O-咖啡酰奎寧酸的方法。方法 色譜柱為島津Interstil ODS柱（250 mm×4.6 mm，5 μm），流動相為乙腈–0.1%三氟乙酸（25∶75），體積流量1.0 mL/min，檢測波長335 nm，柱溫37 ℃，進樣量10 μL。結(jié)果 2種成分均能達到基線分離，重現(xiàn)好，且呈現(xiàn)較好的線性關(guān)系；加樣回收率分別為97.1%、99.8%，RSD值分別為0.66%、0.82%。結(jié)論 該方法準(zhǔn)確、靈敏、可靠，重復(fù)性好，可用于心腦絡(luò)通顆粒的快速檢測。

Objective To develop an HPLC method for simultaneous determination of scutellarin and 4,5-dicaffeoylquinic acid in Xinnao Luotong Granules. Methods The samples were separated on Shimadzu Interstil ODS column (250 mm×4.6 mm, 5 μm) with acetonitrile-0.1% trifluoroacetic acid (25∶75) as mobile phase. The detection wavelength was 335 nm and column temperature was 37 ℃ with injection volume of 10 μL at a flow rate of 0.8 mL/min. Results Two active components were well separated with good repeatability and linearity. The calibration curves were linear. The average recovery rates were 97.1% and 99.8% with RSD values at 0.66% and 0.82%, respectively. Conclusion The method is accurate, sensitive, credible, and repeatable, and could be used for the rapid detection of Xinnao Luotong Granules.