目的 研究丹紅注射液及其單體成分對小鼠小膠質(zhì)細(xì)胞株（BV-2細(xì)胞）的影響及其作用機制。方法 實驗分為對照組、脂多糖（LPS）刺激組、加藥組，檢測不同稀釋倍數(shù)的丹紅注射液、水和醋酸乙酯萃取物及其單體成分對小膠質(zhì)細(xì)胞活力及LPS誘導(dǎo)小膠質(zhì)細(xì)胞一氧化氮（NO）產(chǎn)生的影響。結(jié)果 丹紅注射液及醋酸乙酯萃取物對LPS誘導(dǎo)的小膠質(zhì)細(xì)胞NO的產(chǎn)生沒有抑制作用，但是丹紅注射液水萃取物以及丹酚酸A可以在不影響細(xì)胞活力的情況下抑制NO產(chǎn)生。結(jié)論 丹紅注射液水萃取物中的某些成分以及丹酚酸A抑制激活小膠質(zhì)細(xì)胞NO的產(chǎn)生可能是丹紅注射液神經(jīng)保護(hù)作用的機制之一。

Objective To study the effect and its mechanism of action of Danhong Injection and its monomer components on microglia BV-2 of mice. Methods The experiment was divided into control, lipopolysaccharide (LPS), and dosing groups. The effects of Danhong Injection, water extract, ethyl acetate extract, and monomer compounds on microglia cell viability and LPS-induced NO production were detected. Results Danhong Injection and its ethyl acetate extract had no inhibitory effect on NO production of LPS-induced microglia, but water extract of Danhong Injection and its active component salvianolic acid A affected NO production without affecting cell viability. Conclusion The monomer components from Danhong Injection and salvianolic acid A inhibition NO production in activating microglia may be one of the neuroprotective effect mechanisms for neuroprotection of Danhong Injection.