目的 明確蒿鱉養(yǎng)陰軟堅方對血吸蟲病肝纖維化的治療作用，探討肝星狀細胞凋亡與抗血吸蟲病肝纖維化的關系。方法 血吸蟲尾蚴25條/只經腹壁皮膚感染小鼠，12周之后建立小鼠血吸蟲病肝纖維化模型。將全部感染小鼠隨機分為蒿鱉養(yǎng)陰軟堅方（8.20、2.59、0.82 g/kg）治療組、陽性藥物（復方鱉甲軟肝片組0.54 g/kg、秋水仙堿組0.1 g/kg）組、模型對照組（短模組、長模組）和正常對照組。蒿鱉養(yǎng)陰軟堅方組ig蒿鱉養(yǎng)陰軟堅方干粉狀提取物混懸液；陽性藥物對照組ig相應的陽性藥物；模型組ig生理鹽水，1次/d，共12周。光鏡觀察肝細胞病理變化；鹽酸水解法測定肝細胞羥脯氨酸含量；免疫組化法測定α-SMA表達和ISOL技術測定細胞凋亡情況。結果 蒿鱉養(yǎng)陰軟堅方高、中劑量組膠原蛋白含量均明顯降低（P＜0.05）。模型組HSC細胞α-SMA大量表達；而蒿鱉養(yǎng)陰軟堅方組肝星狀細胞（HSC）細胞α-SMA表達較模型組明顯減少（P＜0.01）。蒿鱉養(yǎng)陰軟堅方中劑量組比模型組肝星狀細胞數(shù)量有顯著差異（P＜0.05），與正常組肝星狀細胞凋亡差異無顯著性（P＞0.05）。結論 蒿鱉養(yǎng)陰軟堅方對小鼠血吸蟲病肝纖維化有治療作用。抑制HSC的活化，并誘導其發(fā)生凋亡，是蒿鱉養(yǎng)陰軟堅方抗血吸蟲病肝纖維化的機制之一。

Objective To explore the therapeutic effect of Haobie Yangyin Ruanjian Prescription on liver fibrosis of schistosomiasis in mice and investigate its mechanism of inducing the apoptosis of hepatic stellate cells (HSC). Methods Every mouse was infected by 25 schistosoma cercarias through skin of abdominal wall, and liver fibrosis was induced after 12 weeks. The infected mice were randomly divided into eight groups: high-, mid-, low-dose (8.20, 2.59, and 0.82 g/kg) Haobie Yangyin Ruanjian Prescription groups, positive control group (Componud Biejia Ruangan Tablet 0.54 g/kg group and colchicine 0.1 g/kg group), model control group (3 months after infection group, 6 months after infection group), and normal control group. Haobie Yangyin Ruanjian Prescription groups and the positive control groups were orally given Haobie Yangyin Ruanjian Prescription and positive control drug powder extractive suspension once daily, respectively. Ig administration of physiological saline to mice in model group once daily lasted for 12 weeks. Hepatic tissue was obtained at the end of the experiment to observe the pathological change under microscope, to determine the content of hydroxyproline (Hyp), and to set out α-SMA expression and cell apoptosis using immunohistochemical method. Results Compared with the model group, collagen content of high- and mid-dose Haobie Yangyin Ruanjian Prescription groups was significantly lower (P<0.05). α-SMA of HSC expressed a lot in the model group, which demonstrated schistosomiasis induced the activation of HSC. α-SMA expression of HSC in the treatment groups was evidently reduced (P<0.01), reminding HSC activation was inhibited. The quantity of HSC in mid-dose HYRP group was significantly different to that in the model group (P<0.05), but had no remarkable difference compared with the normal control group (P>0.05). Conclusion Haobie Yangyin Ruanjian Prescription has the therapeutic effect on the liver fibrosis of schistosomiasis in mice. It could significantly alleviate the degree and range of liver fibrosis,reduce collagen content and α-SMA level of hepatic tissue, and induce HSC apoptosis.