目的 研究從小薊藥材中制備蒙花苷對照品的制備方法和鑒定技術(shù)。方法 采用石油醚脫脂、堿溶解酸沉淀法對70%乙醇小薊藥材提取物進(jìn)行分離純化，通過UV、1H-NMR和13C-NMR進(jìn)行結(jié)構(gòu)鑒定，TLC和HPLC法對產(chǎn)品進(jìn)行純度檢測。結(jié)果 從小薊中分離、純化得到蒙花苷對照品。經(jīng)TLC法檢查無雜質(zhì)斑點(diǎn)，HPLC法檢測質(zhì)量分?jǐn)?shù)達(dá)97%。結(jié)論 該方法制備的蒙花苷對照品符合中藥化學(xué)對照品的相關(guān)要求，可作為小薊藥材及其制劑質(zhì)量控制以及中藥藥效物質(zhì)基礎(chǔ)研究用的化學(xué)對照品。

Objective To investigate the preparation method and identification technique of linarin reference substance from Cirsium setosum. Methods The 70% ethanol extract of C. setosum was isolated and purified with petroleum ether defatting, alkali dissolution and acid deposition. Structural identification was carried out by UV, 1H-NMR, and 13C-NMR, and purities were determined by TLC and HPLC methods. Results Linarin reference substances in C. setosum were isolated and purified. There were no impurity spots by TLC check, and the mass fraction of HPLC detection was up to 97%. Conclusion The linarin reference substance prepared by this method complies with the relevant requirements of the chemical markers in Chinese materia medica (CMM), and could be used as chemical reference substance of C. setosum and quality control for its preparations, as well as the study on the pharmacodynamic material basis of CMM.