目的 研究普通念珠藻多糖分離純化方法，并測定其相對分子質(zhì)量。方法 采用水提醇沉法制備粗多糖，Molish反應(yīng)和硫酸苯酚法對其進行定性和定量檢測，二乙胺基乙基纖維素離子交換柱色譜法進行分離純化，高效凝膠滲透色譜法測定其相對分子質(zhì)量。結(jié)果 普通念珠藻粗多糖的提取率為8.6%，分離獲得一個組成均一的酸性多糖組分NSP，其重均相對分子質(zhì)量為402 717。結(jié)論 NSP為首次從普通念珠藻中分離得到一個酸性多糖組分。

Objective To separate and purify Nostoc commune polysaccharides, and determine their molecular weight. Methods Nostoc commune polysaccharides were obtained by water extraction and alcohol precipitation. After the qualitative and quantitative tests of these crude products by Molish reaction and phenol sulfuric acid method, diethylaminoethyl cellulose (DEAE) ion exchange column chromatography was utilized for their separation and purification. And their molecular weights were determined by high performance gel filtration chromatography (HPGFC). Results The extraction rate of Nostoc commune polysaccharides was 8.6%. After the separation and purification, one homogeneous fraction of NSP was obtained, which was identified as acidic polysaccharides and its average molecular weight was determined to be 402 717. Conclusion NSP is separated from N. commune as acidic polysaccharides for the first time.