目的 建立同時(shí)測定蒼耳丸中連翹酯苷A和綠原酸的HPLC法。方法 采用HPLC法測定。色譜柱：Shim-pack VP-ODS（150 mm×4.6 mm，5 μm）；流動(dòng)相：乙腈–0.2%磷酸溶液，梯度洗脫；體積流量：1.0 mL/min；檢測波長：330 nm；進(jìn)樣量：10 μL；柱溫：40 ℃。結(jié)果 連翹酯苷A在0.232～1.856 μg與峰面積積分值呈現(xiàn)良好的線性關(guān)系（r=0.999 6），平均回收率為97.82%，RSD值為1.55%；綠原酸在0.25～2.00 μg與峰面積積分值呈良好的線性關(guān)系（r=0.999 8），平均回收率為101.1%，RSD值為1.18%。結(jié)論 該方法簡便，結(jié)果準(zhǔn)確，重現(xiàn)性好，可作為蒼耳丸的質(zhì)量控制方法。

Objective To establish a method for the simultaneous determination of forsythoside A and chlorogenic acid in Canger Pill. Methods HPLC method was adopted. A Shim-pack VP-ODS C18 colum (150 mm × 4.6 mm, 5 μm) was adopted with mobile phase consisted of acetonitrile - 0.2% phosphoric acid solution in gradient elution. Detection wavelength was set at 330 nm. The flow rate was 1.0 mL/min. The injection volume was 10 μL at column temperature 40 ℃. Results The linear ranges of forsythoside A and chlorogenic acid were 0.232 — 1.865 μg (r=0.999 6) and 0.25 — 2.00 μg (r=0.999 8), respectively. The average recoveries were 97.82% (RSD=1.55%) and 101.1% (RSD=1.18%), respectively. Conclusion The method is simple, practicable, and accurate, which could be used for the quality control of Canger Pill.