目的 建立HPLC-ELSD法同時測定杜仲中綠原酸和鵝掌楸苷的方法。方法 奧泰C18柱（250 mm×4.6 mm，5 μm）；流動相：乙腈–0.2%醋酸水，梯度洗脫；體積流量：0.9 mL/min；柱溫：35 ℃；漂移管105 ℃，氣體流量：2.5 L/min。結(jié)果 綠原酸和鵝掌楸苷分別在0.021 4～4.280 0 μg和0.020 6～4.120 0 μg呈良好的線性關(guān)系；綠原酸和鵝掌楸苷紫的平均回收率分別為98.57%、103.37%，RSD值分別為2.23%、2.56%。結(jié)論 本法簡便、準確，可用于杜仲中綠原酸和鵝掌楸苷的測定。

Objective To establish the method for the simultaneous determination of chlorogenic acid and liriodendrin in Eucommiae Cortex by HPLC-ELSD. Methods Alltima C18 column (250 mm×4.6 mm，5 μm) was used. The mobile phase consisted of acetonitrile - 0.2% acetic acid solution and the gradient elution was carried out. The flow rate was 0.9 mL/min. The temperature of column and drift tube was set at 35 ℃ and 105 ℃, respectively. The flow rate of gas maintained at 2.5 L/min. Results The linear ranges of chlorogenic acid and liriodendrin were 0.021 4 — 4.280 0 and 0.020 6 — 4.120 0 μg, respectively. The average recovery rates of chlorogenic acid and liriodendrin were 98.57% (RSD=2.23%) and 103.37% (RSD=2.56%). Conclusion The method is simple, convenient, and accurate, and could be used for the determination of chlorogenic acid and liriodendrin in Eucommiae Cortex.