目的 研究葛根正丁醇萃取物的化學(xué)成分。方法 采用聚酰胺柱色譜、反相ODS柱色譜、凝膠柱色譜、制備液相色譜等方法進(jìn)行分離純化，根據(jù)化合物的理化性質(zhì)及波譜數(shù)據(jù)鑒定其結(jié)構(gòu)。結(jié)果 分離鑒定了12個(gè)化合物，分別為葛酚苷元A（1）、葛酚苷元B（2）、染料木素（3）、3′-甲氧基葛根素（4）、3′-羥基葛根素（5）、葛根素芹糖苷（6）、葛根素木糖苷（7）、大豆苷元（8）、大豆素7,4′-二葡萄糖苷（9）、大豆苷（10）、葛根素（11）、胡蘿卜苷（12）。結(jié)論 其中化合物1、2為首次從葛屬植物中分離得到。

Objective To study the chemical constituents in n-buytl alcohol fraction of Puerariae Radix. Methods The compounds were isolated by Polyamide, ODS, and Sephadex LH-20 column chromatographies, and preparative HPLC, and their structures were identified by spectral analyses and physiochemical properties. Results Twelve compounds were isolated and identified as puerol A (1), puerol B (2), genistein (3), 3′-methoxy puerarin (4), 3′-hydroxyl puerarin (5), mirificin (6), puerarin xylosidde (7), daidzein (8), daidzein 7,4′-diglucoside (9), daidzin (10), puerarin (11), and daucosterol (12). Conclusion Compouds 1—2 are isolated from the plants of Pueraria DC. for the first time.