目的 利用脂肪變性肝L-02細胞模型，比較何首烏的生品及不同炮制品（傳統(tǒng)方法炮制品、發(fā)酵炮制品）對脂代謝的干預(yù)作用。方法 將肝細胞傳板至6孔板，分為正常組、模型組、給藥組細胞。模型組與給藥組采用含醫(yī)用脂肪乳的培養(yǎng)液誘導(dǎo)48 h，建立肝細胞脂肪變性模型，給藥組給予何首烏生品及不同炮制品的各濃度水提取物、50%乙醇提取物刺激液進行培養(yǎng)，采用洛伐他汀、非諾貝特為陽性對照。24 h后測定肝細胞內(nèi)三酰甘油（TG）、膽固醇（TC）的量。結(jié)果 與模型組相比，何首烏米根霉發(fā)酵品水提取物及50%乙醇提取物、生何首烏水提取物、黑豆制何首烏50%乙醇提取物和清蒸何首烏50%乙醇提取物在該模型上具有較好的降脂作用。其中何首烏發(fā)酵炮制品的降脂活性尤其明顯，何首烏米根霉發(fā)酵品能極顯著地降低TG的量（P＜0.01）至模型組含量的25%；何首烏米根霉發(fā)酵品水提取物和黑曲霉發(fā)酵品的50%乙醇提取物均可降低TC的量（P＜0.01）至模型組含量的35%。結(jié)論 生何首烏及何首烏傳統(tǒng)炮制品、發(fā)酵炮制品均有顯著地降脂作用，其中由米根霉發(fā)酵得到的發(fā)酵品表現(xiàn)出的較好的降脂活性，表明發(fā)酵法可能成為何首烏炮制法研究的新方向。

Objective To investigate the intervention effects of Polygoni Multiflori Radix (PMR) and its traditional processed and fermented products on the lipid metabolism of steatosis hepatocyte. L-02. Methods The hepatocytes were cultured in 6-well plates and divided into control, model, and treatment groups. The steatosis hepatocyte L-02 model was established by exposure in fat emulsion for 48 h. The hepatocytes in the treatment group were treated with the water extracts and 50% ethanol extract of PMR and its processed products by different way, and lovastatin and fenofibrate were used as positive control. The triacylglycerol (TG) and cholesterin (TC) contents were determined after 24 h. In the same time those were studied in groups. Results Compared with the model group, the water extract and 50% ethanol extract of PMR fermented with Rhizopus oryzae, water extract of PMR, 50% ethanol extract of PMR processed by black bean, and 50% ethanol extract of steaming PMR showed great lipid-lowering effects. PMR fermented with R. oryzae could even significantly reduce the content of TG to about 25% and the content of TC to about 35% in the model group, similar to PMR fermented with Aspergillus niger. Conclusion PMR and its traditionally processed and fermented products show the lipid-lowering effect on TC and TG, especially PMR fermented by R. oryzae. These results indicate the fermentation method may be a new research processing method of PMR.