目的 通過(guò)觀察痛風(fēng)顆粒各部位對(duì)高尿酸血癥大鼠血尿酸、尿尿酸、血黃嘌呤氧化酶活性和肝臟黃嘌呤氧化酶活性的影響，探討其治療痛風(fēng)的物質(zhì)基礎(chǔ)和機(jī)制。方法 以腺嘌呤合乙胺丁醇法誘導(dǎo)大鼠高尿酸血癥模型，分別用磷鎢酸法和酶比色法檢測(cè)尿酸和黃嘌呤氧化酶的含量。結(jié)果 黃酮類成分在降尿酸和抑制黃嘌呤氧化酶活性上均起主要作用；生物堿類成分對(duì)尿中尿酸的排泄和血清黃嘌呤氧化酶活性的抑制起較重要作用，有機(jī)酸類成分均未表現(xiàn)出明顯作用；全方和有效部位組合有明顯的降尿酸和抑制黃嘌呤氧化酶活性的作用。結(jié)論 黃酮類、生物堿和有機(jī)酸類有效部位組合后的藥效與處方藥一致，是該處方的有效部位群，對(duì)高尿酸血癥模型大鼠表現(xiàn)出的降尿酸和抑制黃嘌呤氧化酶活性的作用最為顯著。

Objective To discuss the material foundation and mechanism of Tongfeng Granule by observing the effects of various fractions in Tongfeng Granule on the blood uric acid (UA), urinary UA, blood xanthine oxidase (XOD) activity and liver XOD activity of hyperuricemic rats. Methods The hyperuricemia rat model was established by adenine and ethambutol, and the contents of UA and XOD were determined with phosphotungstic acid method and enzyme colorimetric method, respectively. Results The flavonoids played the leading roles in dropping UA and inhibition on XOD activity; The alkaloids in the excretion of UA urine and restraining serum SOD activity played the more important role, but organic acids had no obvious effect. Prescription and effective parts combination had obvious activitied in dropping UA and inhibition on XOD activity Conclusion The effective parts of orthogonal design research shows that the combination of flavonoids, organic acids, and alkaloids is the strongest to anti gout. A combination (flavonoids, alkaloids, and organic acid) shows the active role in droping UA and inhibition of XOD in hyperuricemia model rats.