目的 建立一測(cè)多評(píng)法同時(shí)定量測(cè)定烏頭類藥材中6種烏頭類生物堿。方法 采用高效液相色譜法同時(shí)測(cè)定烏頭類藥材中6種生物堿，以烏頭堿為內(nèi)參物，測(cè)定其與次烏頭堿、新烏頭堿、苯甲酰烏頭原堿、苯甲酰次烏頭原堿、苯甲酰新烏頭原堿的相對(duì)校正因子，利用烏頭堿和相對(duì)校正因子對(duì)其他5種生物堿成分進(jìn)行測(cè)定，同時(shí)利用外標(biāo)法測(cè)定這6種成分，比較兩種測(cè)定方法的差異，驗(yàn)證一測(cè)多評(píng)法的可行性和準(zhǔn)確性。結(jié)果 在一定線性范圍內(nèi)，烏頭堿與次烏頭堿、新烏頭堿、苯甲酰烏頭原堿、苯甲酰次烏頭原堿和苯甲酰新烏頭原堿的相對(duì)校正因子分別為0.780、1.008、0.836、0.907、0.987，在不同實(shí)驗(yàn)條件下相對(duì)校正因子重現(xiàn)性良好，不同烏頭類藥材中6種生物堿成分含量計(jì)算值與實(shí)測(cè)值間無(wú)顯著性差異。結(jié)論 一測(cè)多評(píng)法在烏頭類藥材及制劑質(zhì)量控制中應(yīng)用是可行的、準(zhǔn)確的。

Objective To establish a quantitative analysis of multi-components by single marker (QAMS) for the simultaneous determination of six alkaloids in the herbs of Aconitum L. species. Methods An HPLC method was established to determine the six alkaloids (aconitine, hypaconitine, mesaconitine, benzoylaconine, benzoylhypaconine, and benzoylmesaconine). Using aconitine and five relative correction factors (RCFs), the contents of the five other alkaloids in all samples were calculated by their RCFs, which were compared with those determined by the external standard method. The feasibility and accuracy of QAMS method were verified. Results Within a certain range, the RCFs of hypaconitine, mesaconitine, benzoylaconine, benzoylhypaconine, and benzoylmesaconine to aconitine were 0.780, 1.008, 0.836, 0.907, and 0.987, respectively, with the good repeatability in different experimental conditions. There was no significant difference between the QAMS method and the external standard method. Conclusion The QAMS method is feasible and accurate for the quality control of Aconitum L. species and their preparations.