[關鍵詞]
[摘要]
目的 考察枸杞提取液對球形紅細菌生長的影響。方法 在不同質量濃度的常規(guī)培養(yǎng)基中添加不同質量濃度的枸杞提取液,培養(yǎng)球形紅細菌,比較球形紅細菌的活菌數、生長曲線和脫氫酶活性,考察枸杞提取液對球形紅細菌生長的影響。結果 在培養(yǎng)基中添加枸杞提取液可縮短球形紅細菌生長延遲期,使其提前進入指數期和穩(wěn)定期;當質量濃度為12.5 g/L時,球形紅細菌活菌數增加到之前的1.5倍,使球形紅細菌脫氫酶活性提高到之前的3.3倍。結論 培養(yǎng)基中添加一定量的枸杞提取液可以促進球形紅細菌的生長,使球形紅細菌生長周期縮短、活力提高。
[Key word]
[Abstract]
Objective To study the effects of Lycium barbarum extract on the growth of Rhodobacter sphaeroides. Methods Adding L. barbarum extract into general culture medium at different concentration, the effects of L. barbarum extract on the growth of R. sphaeroides were investigated by culturing R. sphaeroide and comparing living R. sphaeroides bacteria number, growth curve, and dehydrogenase activity.. Results After adding L. barbarum extract into the culture medium, the growth delay period of R. sphaeroides was shortened and the expotential and stationary periods were advanced. When the concentration of L. barbarum extract was 12.5 g/L, the number of living R. sphaeroides was increased in 1.5 times, and the dehydrogenase activity of R. sphaeroides was improved in 3.3 times as much as before. Conclusion When L. barbarum extract is added into the culture medium, the growth and the activity of R. sphaeroides could be improved, and the growth cycle could be shortened.
[中圖分類號]
[基金項目]
國家自然科學基金資助項目(81173473);山西省自然科學基金資助項目(2010011048-2);太原市科技項目人才專項明星專題項目(120247-08);山西醫(yī)科大學青年基金(02201128,02201124)