目的 通過生物酶解技術(shù)優(yōu)化北蒼術(shù)多糖的酶輔助超聲提取方法，并考察其體外抗腫瘤活性。方法 選用纖維素酶和果膠酶水解北蒼術(shù)藥材，酶解后采用超聲法提取北蒼術(shù)藥材中多糖。以多糖得率為指標(biāo)，在單因素試驗(yàn)的基礎(chǔ)上采用4個(gè)因素（酶用量、酶解時(shí)間、酶解pH值、酶解溫度）3個(gè)水平L9(34)正交設(shè)計(jì)法對(duì)酶解條件進(jìn)行優(yōu)選，并選用苯酚–濃硫酸法測(cè)定多糖，采用MTS、臺(tái)盼藍(lán)法對(duì)北蒼術(shù)粗多糖體外抗腫瘤活性進(jìn)行初探。結(jié)果 最佳酶解條件為酶用量為藥材1.2%、在60 ℃、pH 5條件下酶解50 min，再以超聲提取40 min，北蒼術(shù)多糖的得率為32.29%，所提粗多糖對(duì)HeLa細(xì)胞增殖的抑制率為64.42%。結(jié)論 酶解輔助超聲提取北蒼術(shù)中多糖簡(jiǎn)便易行，可以提高多糖得率，多糖活性較好。

Objective To optimize the enzymatic auxiliary ultrasonic extraction of polysaccharide from Atractylodes chinensis by biological enzyme technology and to study its antitumor activity in vitro. Methods Cellulase and pectinase were used for hydrolysis and then ultrasonic extracting method was chosen to extract the polysaccharide from roots of A. chinensis. The yield of polysaccharide purity from crude polysaccharide was taken as indicators. Based on single-factor test, the ultrasonic-assisted extracting technology of A. chinensis polysaccharide was optimized by L9 (34) orthogonal test, and four factors included enzymolysis temperature, enzyme content, enzymolysis pH value, and enzymolysis time with three levels. The phenol-sulphuric acid method was applied for the determination of polysaccharide, and then using MTS and trypan blue staining to study antitumor activity on the polysaccharides. Results The optimal enzymolysis extracting conditions of A. chinensis polysaccharide were as follows: temperature was 50 ℃, content was 1.2%, pH value was 5 , and time was 50 min. Under the conditions, the purity of polysaccharide was 32.29%, and inhibitory rate of polysaccharide on the proliferation of HeLa cells was 64.42%. Conclusion Enzymatic auxiliary for ultrasonic extraction process has the advantages of short time, simple operation, and higher extract rate, which could provide the reference for development and utilization of A. chinensis.

天津市科技攻關(guān)項(xiàng)目（06YFGPSH02900）